TABLE 4.

Effect of number of Phe+ colonies on selective plates on further accumulation of mutants in wild-type and MutY-defective P. putida strainsa

DayMean no. of Phe+ colonies ± SD at initial inocolum:
5 × 108 cells5 × 107 cells5 × 106 cells
Wild typemutYWild typemutYWild typemutY
22.0 ± 1.7260 ± 108030 ± 1303.0 ± 1.8
32.3 ± 1.7423 ± 33092 ± 13010 ± 2.6
49.3 ± 4.5108 ± 301.8 ± 0.5183 ± 29023 ± 2.6
519 ± 9.837 ± 150.8 ± 0.9113 ± 43025 ± 5.1
641 ± 208.3 ± 2.51.8 ± 2.422 ± 11060 ± 6.5
739 ± 215.2 ± 1.92.8 ± 2.015 ± 6.7072 ± 9.6
  • a Different amounts of pEST1414-carrying cells (5 × 108, 5 × 107, or 5 × 106) were plated with a constant number (109) of scavenger cells onto phenol minimal plates. Results of four separate platings are shown.