TABLE 2.

Modified nucleoside levels for parent strain MC1061, iscS mutant CL100(DE3), and CL100(DE3) complemented with plasmid pCL010a

StrainRelative level
s2Cmnm5Umnm5s2Us4Ui6Ams2i6A
MC10611.0 (0.2)(0.015)1.0 (0.11)1.0 (1.2)NDb(0.52)
CL100(DE3)0.02(0.15)NDND(0.65)ND
CL100(DE3)/pCL0100.9(0.01)0.71.0ND(0.47)
  • a Levels of 2-thiocytidine (s2C), 5-methylaminomethyl-2-thiouridine (mnm5s2U), and 4-thiouridine (s4U) were measured as the area ratio to pseudouridine (Ψ) and then normalized relative to the parent strain set at 1.0. Parenthetical values were not normalized. Levels of 6-N-dimethylalyl-2-methylthioadenosine (ms2i6A) and i6A are reported relative to t6A and were not normalized to the parent strain. All nucleosides were quantified at 260 nm except 4-thiouridine, which was quantitated at 330 mm.

  • b ND, none detected.