TABLE 3.

Drug resistance of E. coli KAM3 cells harboring combinations of plasmids carrying mdtA and mdtBCa

DrugMIC (μg/ml) against KAM3 cells harboring combinations of plasmids:
pTrcmdtCpACYCmdtA and pTrcmdtCpTrcmdtBCpACYCmdtA and pTrcmdtBC
−IPTG+IPTG−IPTG+IPTG−IPTG+IPTG−IPTG+IPTG
Deoxycholate2,0002,0008,0008,0001,0002,0004,00032,000
Cholate16,00016,00032,00064,0008,00016,00016,00064,000
Taurocholate32,00032,00032,00064,00032,00032,00064,000>64,000
SDS64646464646464128
Novobiocin11111128
  • a MICs more than fourfold higher than those against host KAM3 cells are indicated by boldface. The gene expression from pTrc plasmids was induced with 10 μM IPTG (isopropyl-β-d-thiogalactopyranoside).