TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype and/or phenotypeSource or reference
Strains
    E. coli
        DH5αλpirF Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 λ::pir10, 14
        Sm10λpirthi recA thr leu tonA lacY supE RP4-2-Tc::Mu λ::pirLaboratory strain
        AC-E479Sm10λpir(pSL479) Apr16
        AC-E481Sm10λpir(pSL481) Apr16
        AC-E482Sm10λpir(pSL482) AprThis work
        AC-E1078Sm10λpir(pSL148) AprThis work
        AC-E1082Sm10λpir(pSL108) AprThis work
        AC-E1211Sm10λpir(pAT1211) AprThis work
    V. cholerae El Tor biotype
        AC-V66C6709-1 lacZ::res-tet-res Smr Tcr2
        Bah-2E7946 ΔattRS Smr lacZ22
        AC-V282C6709-1 ΔvieSAB Smr Tcr17
        AC-V279AC-V66 ΔvieAB Smr Tcr17
        AC-V494AC-V66 ΔtoxT Smr Tcr16
        AC-V752AC-V66 vieS::mTn5Kn2 Smr Tcr Knr18
        AC-V765AC-V66 ΔvieSAB Smr TcrThis work
        AC-V323bAC-V66 ΔvieB Smr TcrThis work
        AC-V1079AC-V66 ΔvieS Smr TcrThis work
        AC-V1221AC-V66 ΔvieA-HTH Smr TcrThis work
        AC-V553AC-V66 ctxA::tnpR135 Smr Tcr Apr16
        AC-V1023AC-V752 ctxA::tnpR135 Smr Tcr Apr18
        AC-V1083AC-V323b ctxA::tnpR135 Smr Tcr AprThis work
        AC-V1097AC-V66 ctxA::tnpR168 Smr Tcr AprThis work
        AC-V1098AC-V752 ctxA::tnpR168 Smr Tcr AprThis work
        AC-V1151AC-V1079 ctxA::tnpR135 Smr Tcr AprThis work
        AC-V1093AC-V765 tcpA::tnpR135 Smr Tcr AprThis work
        AC-V1117AC-V66 pMMB67EH Smr Tcr AprThis work
        AC-V1110AC-V765 pMMB67EH Smr Tcr AprThis work
        AC-V1118AC-V66 pAT1114 Smr Tcr AprThis work
        AC-V1120AC-V765 pAT1114 Smr Tcr AprThis work
Plasmids
    pIVET5oriR6K mobRP4 tnpR-lacZY Apr2
    pSL479pIVET5::tcpA′::tnpR13516
    pSL481pIVET5::ctxA′::tnpR13516
    pSL482pIVET5::ctxA′::tnpR168This work
    pCVD442oriR6K mobRP4 sacB Apr6
    pSL108pCVD442::ΔvieB17
    pSL148pCVD442::ΔvieSThis work
    pAT1211pCVD442::ΔvieA-HTHThis work
    pCR-Scriptf1(+) ori AprStratagene
    pMMB67EHIncQ broad-host-range cloning vector, Apr21
    pAT1113pCR-Script::vieSABThis work
    pAT1114pMMB67EH::vieSABThis work
    pGEM-Tf1 ori AprPromega
    pAT853pGEM-T::′ctxAThis work
    pAT854pGEM-T::′ctxBThis work
    pAT856pGEM-T::′rpoBThis work
    pDSM701pGEM-T::′toxTThis work