TABLE 2.

Similarity of gene products from 2,4-DNT gene cluster to selected homologsa

Gene productSourceFunctionLength (no. of aa)% IdentityAlignmentSource or reference
DntAaBurkholderia cepacia R342,4-DNT dioxygenase328
NtdAaPseudomonas sp. strain JS422-Nitrotoluene dioxygenase328991-328—1-32848
NagAaRalstonia sp. strain U2Naphthalene dioxygenase328991-328—1-32817
DntAaBurkholderia sp. strain DNT2,4-DNT dioxygenase346981-293—1-29368
NahAaPseudomonas putida 9816Naphthalene dioxygenase328661-328—1-29364
ORF1aBurkholderia cepacia R34302
NagGRalstonia sp. strain U2Salicylate hydroxylase423991-295—1-29517
ORF2Pseudomonas sp. strain JS42186991-179—1-17948
ORF2Burkholderia sp. strain DNT423931-295—1-29568
OhbBPseudomonas aeruginosa JB2o-Halobenzoate dioxygenase4217910-292—7-290Accession no. AAC69484
OhbBPseudomonas aeruginosa 142o-Halobenzoate dioxygenase4285120-257—12-24577
IpbAaPseudomonas putida RE204Isopropylbenzene dioxygenase4593730-226—41-23614
ORF1bBurkholderia cepacia R34120
NagGRalstonia sp. strain U2Salicylate hydroxylase4231001-120—304-42317
ORF2Burkholderia sp. strain DNT4231001-120—304-42368
OhbBPseudomonas aeruginosa JB2o-Halobenzoate dioxygenase421811-118—304-423Accession no. AAC69484
OhbBPseudomonas aeruginosa I42o-Halobenzoate dioxygenase428363-119—294-41077
IsbAaPseudomonas putida RE204Isopropylbenzene dioxygenase459253-120—325-45114
ORF2Burkholderia cepacia R34161
ORFXBurkholderia sp. strain DNT1611001-161—1-16168
NagHRalstonia sp. strain U2Salicylate hydroxylase161981-161—1-16117
OhbCPseudomonas aeruginosa JB2o-Halobenzoate dioxygenase157571-161—1-157Accession no. AAC69484
OhbAPseudomonas aeruginosa I42o-Halobenzoate dioxygenase176268-161—27-17677
DntAbBurkholderia cepacia R342,4-DNT dioxygenase104
DntAbBurkholderia sp. strain DNT2,4-DNT dioxygenase104861-104—1-10468
NtdAbPseudomonas sp. strain JS422-Nitrotoluene dioxygenase104861-104—10448
NagAbRalstonia sp. strain U2Naphthalene dioxygenase104841-104—1-10417
NahAbPseudomonas putida 9816Naphthalene dioxygenase104741-103—1-10364
DntAcBurkholderia cepacia R342,4-DNT dioxygenase447
DntAcBurkholderia sp. strain DNT2,4-DNT dioxygenase451931-447—6-45168
NagAcRalstonia sp. strain U2Naphthalene dioxygenase447911-447—1-44717
NtdAcPseudomonas sp. strain JS422-Nitrotoluene dioxygenase447901-447—1-44748
NahAcPseudomonas putida 9816Naphthalene dioxygenase449831-447—1-44964
DntAdBurkholderia cepacia R342,4-DNT dioxygenase194
DntAdBurkholderia sp. strain DNT2,4-DNT dioxygenase194981-194—1-19468
NagAdRalstonia sp. strain U2Naphthalene dioxygenase194921-194—1-19417
NtdAdPseudomonas sp. strain JS422-Nitrotoluene dioxygenase194921-194—1-19448
NahAdPseudomonas putida 9816Naphthalene dioxygenase194781-194—1-19464
DntBBurkholderia cepacia R344-Methyl-5-nitrocatechol oxygenase560
DntBBurkholderia sp. strain DNT4-Methyl-5-nitrocatechol oxygenase5485315-547—12-54722
PheAPlasmid pEST126Phenol monooxygenase610315-546—24-59846
TfdBPseudomonas putida (pEST401)2,4-dichlorophenol hydroxylase5853115-527—9-56332
TfdBIIRalstonia eutropha JMP134Chlorophenol monooxygenase5863013-547—7-58439
ORF3Burkholderia cepacia R34Intradiol dioxygenase278
HadCRalstonia pickettii DTP0602Hydroxyquinol-1,2-dioxygenase315651-264—1-26472
TfdCAlcaligenes eutrophusChlorocatechol 1,2-dioxygenase2552922-278—2-25018
DntDBurkholderia cepacia R34Trihydroxytoluene oxygenase31428
DntDBurkholderia sp. strain DNTTrihydroxytoluene oxygenase315601-313—1-31421
BphCPseudomonas stutzeri A4012,3-Dihydroxybiphenyl dioxygenase363386-308—28-32970
HpcABrucella melitensisHomoprotocatechuate dioxygenase3312514-308—30-30911
XylEPseudomonas putida pWWOCatechol dioxygenase3072123-279—20-28541
DntEBurkholderia cepacia R34Methylmalonate semialdehyde dehydrogenase508
MmsARattus norvegicusMethylmalonate semialdehyde dehydrogenase535609-494—34-51931
MmsAPseudomonas aeruginosaMethylmalonate semialdehyde dehydrogenase4975515-497—5-48766
CmtCPseudomonas putida F1p-Cumic aldehyde dehydrogenase4943418-489—19-49013
StyDPseudomonas sp. strain Y2Phenylacetaldehyde dehydrogenase5023116-487—22-49878
ORF13Burkholderia cepacia R34Putative NAD(P)H-dependent reductase188
ORF9Burkholderia cepacia R34Putative NAD(P)H-dependent reductase189625-184—7-186
LanZ4Streptomyces cyanogenusPutative reductase2003842-156—55-16380
SsuEPseudomonas putida S-313NADH-dependent FMN reductase200295-135—3-133Accession no. O85762
DntGBurkholderia cepacia R342,4-Dihydroxy-5-methyl-6-oxo-2,4-hexadienoate isomerase/4-hydroxy-2-keto-5-methyl-6-oxo-3-hexenoate hydrolase281
HpcEMethanococcus jannaschii2-Hydroxyhepta-2,4-diene-1,7-dioate (HHDD) isomerase2374545-256—36-2367
HpcEBrucella melitensisHHDD isomerase/5-carboxymethyl-2-oxo-3-hexene- 1,7-dioate decarboxylase311381-279—25-30911
DitDPseudomonas abietaniphilaPutative isomerase2933540-255—60-27440
ORF10Burkholderia cepacia R34Putative maleylacetate reductase379
TftEBurkholderia cepacia AC1100Maleylacetate reductase352658-338—4-33210
ClcEPseudomonas sp. strain B13Maleylacetate reductase352537-336—2-32930
TfdFAlcaligenes eutrophus (pJP4)Maleylacetate reductase3545216-338—12-33252
ORF12Burkholderia cepacia R34Putative regulatory protein301
NahRPseudomonas putida G7Regulatory protein300611-300—1-30063
OhbRPseudomonas putida JB2Putative regulatory protein310571-301—1-301Accession no. AAC69484
  • a Deduced amino acid sequences of gene products from R34 were compared to previously described peptides using the BlastP search algorithm. Length indicates size of peptide (in amino acids). % Identity, percent of identical amino acids over the aligned region. Alignment, first range indicates portion of the R34-derived peptide and second range indicates portion of homolog used in alignment.