TABLE 1.

Bacterial strains

StrainRelevant genotype or plasmidReference
E. coli
    SM10Conjugation donor; F thi-1 thr-1 leuB6 recA tonA21 lacY1 supE44 (MuC+) λ [RP4-2(Tc::Mu)] Kmr Tra+44
    XL1-BluePlasmid manipulations, recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)]Stratagene
    DP-E4067Integration vector, pPL1/SM10This study
    DP-E4068hly integration vector, pPL24/SM10This study
    DP-E4069actA integration vector, pPL25/SM10This study
    DP-E4190Integration vector, pPL2/SM10This study
L. monocytogenes
    10403SWild type3
    DP-L405610403S, phage curedThis study
    DP-L4027DP-L2161, phage cured, ΔhlyThis study (22)
    DP-L4029DP-L3078, phage cured, ΔactAThis study (46)
    DP-L4074DP-L4056 comK::pPL1This study
    DP-L4075DP-L4027 Δhly comK::pPL24This study
    DP-L4076DP-L4056 comK::pPL24This study
    DP-L4077DP-L4029 ΔactA comK::pPL25This study
    DP-L4078DP-L4056 comK::pPL25This study
    SLCC-5764Virulence gene overexpresser (Mack, DP-L861)27
    DP-L862Mack-4R (SLCC-5764 rough isolate)S. Katheriou
    DP-L4082SLCC-5764, Strr derivativeThis study
    DP-L3780SLCC-5764 ΔactA (deletion of amino acids 7-633)This study
    DP-L4083DP-L3780, Strr derivativeThis study
    DP-L4084DP-L4082 comK::pPL1This study
    DP-L4085DP-L4082 comK::pPL25This study
    DP-L4086DP-L4083 comK::pPL1This study
    DP-L4087DP-L4083 ΔactA comK::actAThis study
    DP-L4088DP-L1169S 4b strain, StrrThis study
    DP-L4089DP-L1172S 4b strain, StrrThis study
    DP-L4090DP-L4088 comK::pPL1This study
    DP-L4091DP-L4089 comK::pPL1This study
    DP-L4199EGDe, Strr derivativeThis study
    DP-L4026WSLC 1042 (ATCC 23074)31
    DP-L4061WSLC 1042::PSAThis study
    DP-L422110403S, tRNAArg::pPL2This study
    DP-L4379LO28, Strr derivativeThis study
L. innocua
    DP-L4391CLIP 11262, Strr derivative15