TABLE 1.

Plasmids used in this study

PlasmidDescriptionReference
pGEM-T EasyCloning vector, AprPromega
pBluescript II KSCloning vector, AprStratagene
pLDR8 int gene expression vector, Kmr 9
pLDR9Cloning vector for integration into the λ-attB, Kmr 9
pLDR11Cloning vector for integration into the λ-attB, Tcr 9
pGWB1917 wb*Nissle 1917 cloned into pGEM-T Easy, AprThis work
pGWB536 wb*536 cloned into pGEM-T Easy, AprThis work
pBWB536 wb*536 cloned into pBluescript II KS, AprThis work
pGLG25042,504 bp of the 5′-proximal region of wb*536 (including JUMPstart region, wzx536, and the 986-bp fragment of wzy536) cloned into pGEM-T Easy, AprThis work
pBLG25042,504 bp of the 5′-proximal region of wb*536 (including JUMPstart region, wzx536, and the 986-bp fragment of wzy536) cloned into pBluescript II KS, AprThis work
pGLG28492,849 bp of the 5′-proximal region of wb*536 (including JUMPstart region, wzx536, and the 1,343-bp intact wzy536) cloned into pGEM-T Easy, AprThis work
pBLG28492,849 bp of the 5′-proximal region of wb*536 (including JUMPstart region, wzx536, and the 1,343-bp intact wzy536) cloned into pBluescript II KS, AprThis work
pLBW1Pbla::wzy536 cloned into pLDR11, TcrThis work
pGPW1Pwb*Nissle 1917::wzy536 cloned into pGEM-T Easy, AprThis work
pLPW1Pwb*Nissle 1917::wzy536 cloned into pLDR9, KmrThis work