TABLE 2.

Effects of deletion mutations in divJ on DivJ-GFP localization, growth, and viabilitya

StrainRelevant genotypeDivJ-GFP foci at stalk poles (%)bDoubling time (min)Relative viability
CB15Parent strainNA1051.0
PC9808CB15/pTR100NA1180.71
PC3017divJ::ΩNA1230.56
PC9809divJ::Ω/pTR100NA1690.42
PC9810divJ::Ω/pdivJ-gfp721240.68
PC9814divJ::Ω/pdivJ1-1668-gfp (DivJ1-557)751160.60
PC9812divJ::Ω/pdivJ1-975-gfp (DivJ1-326)661520.40
PC9813divJ::Ω/pdivJ1-735-gfp (DivJ1-245)<11680.40
PC9879divJ::Ω/pdivJΔ811-936 (DivJΔ271-312)41240.62
PC9880divJ::Ω/pdivJΔ751-936 (DivJΔ251-312)21230.62
  • a Strains were grown and plated on PYE medium with antibiotics as appropriate. Results presented are the averages from three to five experiments. For DivJ-GFP scoring, over 200 cells were scored per strain. See Materials and Methods for details.

  • b NA, not applicable.