TABLE 1.

Bacterial strains and plasmids

DesignationPropertyReference
Y. enterocolitica strains
    W22703O:9 serotype; pYVe227 Nalr; wild-type isolate14
    CT133Δ(lcrH) Nalr2
    EC2Δ(yscM1 yscM2) Nalr7
    EC3Δ(yopD lcrH) NalrThis study
    EC4Δ(yopD yscM1 yscM2) NalrThis study
    EC5Δ(yscM2) Nalr7
    EC6Δ(yopD lcrH yscM1 yscM2) NalrThis study
    EC7Δ(lcrH yscM1 yscM2) NalrThis study
    MC3Δ(yopQ) Nalr3
    VTL1Δ(yopN) Nalr36
    VTL2Δ(yopD) Nalr38
E. coli strains
    DH5αsupE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA128
    S17-1λpir+16
Plasmids
    pCT129pLC28 derivative, lcrH deletion construct2
    pDA183yopQ pro-QUTR-npt Cmr3
    pDA184yopQ pro-QUTR-yopQ (1-15aa)-npt Cmr3
    pDA208yopQ pro-QUTR-yopQ (1-10aa)-npt Cmr3
    pDA209yopQ pro-QUTR-yopQ-nptUTR-npt Cmr3
    pDA218yopQ pro-QUTR-yopQ Cmr3
    pDA219tac promoter fused to yopQ; Cmr3
    pDA243yopQ pro-QUTR-yopQ-npt Cmr3
    pDA259tac promoter fused to gst; Cmr7
    pDA325tac promoter fused to lcrH; Cmr2
    pDA330yopQ pro-nptUTR-npt Cmr2
    pEC52yopQ pro-QUTR(178-148)-nptUTR-npt CmrThis study
    pEC53yopQ pro-QUTR(178-120)-nptUTR-npt CmrThis study
    pEC54yopQ pro-QUTR(178-88)-nptUTR-npt CmrThis study
    pEC55yopQ pro-QUTR(178-58)-nptUTR-npt CmrThis study
    pEC56yopQ pro-QUTR(178-28)-nptUTR-npt CmrThis study
    pEC57yopQ pro-QUTR(178-12)-nptUTR-npt CmrThis study
    pEC58yopQ pro-QUTR(178-1)-nptUTR-npt CmrThis study
    pEC70yopQ pro-QUTR(150-1)-nptUTR-npt CmrThis study
    pEC71yopQ pro-QUTR(120-1)-nptUTR-npt CmrThis study
    pEC72yopQ pro-QUTR(90-1)-nptUTR-npt CmrThis study
    pEC73yopQ pro-QUTR(60-1)-nptUTR-npt CmrThis study
    pEC74yopQ pro-QUTR(30-1)-nptUTR-npt CmrThis study
    pEC80yopQ pro-yopEUTR-npt CmrThis study
    pEC83npt pro-nptUTR-npt CmrThis study
    pEC84npt pro-QUTR-npt CmrThis study
    pEC102yopE pro-nptUTR-npt CmrThis study
    pEC112yopQ pro-nptUTR(yopEUTR hyb)-npt CmrThis study
    pEC138yopQ pro-yopEUTR(A11C)-npt CmrThis study
    pEC139yopQ pro-yopEUTR(A10C)-npt CmrThis study
    pEC140yopQ pro-yopEUTR(T9G)-npt CmrThis study
    pEC141yopQ pro-yopEUTR(A8C)-npt CmrThis study
    pEC142yopQ pro-yopEUTR(A7C)-npt CmrThis study
    pEC143yopQ pro-yopEUTR(A6C)-npt CmrThis study
    pEC144yopQ pro-yopEUTR(T5G)-npt CmrThis study
    pEC145yopQ pro-yopEUTR Δ(11-5)-npt CmrThis study
    pEC148yopQ pro-nptUTR(yopQUTR hyb)-npt CmrThis study
    pEC260tac promoter fused to gst; Tetr7
    pEC345tac promoter fused to yscM1; Cmr7
    pEC346tac promoter fused to gst-yscM1; Cmr7
    pEC347tac promoter fused to gst-yscM1; Tetr7
    pEC348tac promoter fused to yscM2; Cmr7
    pEC349tac promoter fused to gst-yscM2; Cmr7
    pEC350tac promoter fused to gst-yscM2; Tetr7
    pKR12tac promoter fused to yopD; lcrH CmrThis study
    pVLΔyopDpLC28 derivative; yopD deletion construct38