Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidCharacteristic(s)Reference(s) or source
    M. gryphiswaldense
        MSR-1 R3/S1WT (spontaneous Rifr Smr mutant)37
        MSR-1 IK-1R3/S1 ΔrecAThis study
    E. coli
        DH5αF φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK mK+) phoA supE44 λ thi-1 gyrA96 relA1Invitrogen
        BW29427dap mutant auxotrophic derivative of E. coli strain B2155K. Datsenko and B. L. Wanner, unpublished data
    pJet 1.2/bluntApr, eco47IR (lethal restriction enzyme gene), rep (pMB-1)Fermentas
    pJetIB013+014pJet containing the upstream fragment of the recA geneThis study
    pJetIB015+016pJet containing the downstream fragment of the recA geneThis study
    pCM184Apr Kmr Tetr; broad-host-range allelic exchange vector20
    pCM184+recAflankpCM184 with 1.6- and 1.8-kb up- and downstream fragments of recAThis study
    pCM157Tetr; Cre recombinase expression vector20
    pBBR1MCS5Kmr; mobilizable broad-host-range vector19
    pBBR1MCS5recApBBR1MCS5 containing recAThis study
    pAL01Kmr; pK19mobGII vector (Kmr, pMB-1 replicon, gusA, lacZ) containing a 2-kb fragment upstream of mgr4019A. Lohße, unpublished data