Table 2.

Results of PCR amplification of various regions within the mamAB operon from the spontaneous Mag mutants of the WT straina

ClonePresence of following amplification product:
mamH-mamE (3.8 kb)mamJ-mamL (2.7 kb)mamM-mamN (2.2 kb)mamO-mamA (3.4 kb)mamQ-mamS (2.5 kb)mamT-mamU (1.4 kb)
1+
3++
4++
6+++
7+++++
80+++++
9+++
10+0++++
11+++++
12+0++++
13++++++
14++++++
150++++
16++++++
19++++++
20++++++
22++++++
23++++++
  • a Six regions were probed by PCR. The presence of bands was detected by agarose gel electrophoresis (see Fig. S2 in the supplemental material). −, no product detectable; +, product detectable; 0, product of aberrant size detectable.