Table 1.

Bacillus subtilis strains used in this study

StrainGenotypeSource or referencea
168trpC2Laboratory collection
GP193ΩymdA::pGP774 (PxylA-rny cat)11
GP1012trpC2 rny-Strep ΔymdB::catSee experimental procedures in text
GP1013trpC2 rny-Strep ΔymdB::cat amyE::pGP1354 (Ppgk::ymdB)pGP1354 → GP1012
GP1016trpC2 rny-Strep ΔymdB::cat amyE::pGP1354 (Ppgk::ymdB) pnpA-3×FLAGpGP1376 → GP1013
GP1022trpC2 rny-Strep ΔymdB::cat amyE::pGP1354 (Ppgk::ymdB) cshA-3×FLAGpGP1333 → GP1013
GP1023trpC2 rny-Strep ΔymdB::cat amyE::pGP1354 (Ppgk::ymdB) pfkA-3×FLAGpGP1375 → GP1013
GP1024trpC2 rny-Strep ΔymdB::cat amyE::pGP1354 (Ppgk::ymdB) rnjA-3×FLAGpGP1376 → GP1013
GP1025trpC2 rny-Strep ΔymdB::cat amyE::pGP1354 (Ppgk::ymdB) ytsJ-3×FLAGpGP1758 → GP1013
GP1033trpC2 rny-Strep spcpGP1391 → 168
GP1039trpC2 rny-Strep ΔymdB::cat amyE::pGP1354 (Ppgk::ymdB) rnjB-3×FLAGpGP1851 → GP1013
GP1040trpC2 rny-Strep ΔymdB::cat amyE::pGP1354 (Ppgk::ymdB) eno-3×FLAGpGP1264 → GP1013
GP1091trpC2 lacA::xylR aphA3pGP884 → 168
GP1092trpC2 lacA::xylR aphA3 ΩymdA::pGP774 (PxylA-rny cat)pGP774 → GP1091
  • a Arrows indicate construction by transformation.