Strains and plasmids

Strain or plasmidRelevant characteristic(s)Reference or source
    pTA131Ampr; pBluescript II with BamHI-XbaI fragments from pGB70 harboring pfdx-pyrE222
    pTA231Ampr; pBluescript II with pHV2 replication origin harboring pfdx-trpA22
    pTA963Ampr, pyrE2 and hdrB markers, Trp-inducible (ptna) promoter24
    pAF9pTA963 carrying artA14
    pFH26pTA131 carrying fragment containing nucleotides encoding PFG with 400 bp flanking the intergenic region of slg and hvo_2073; the trpA gene cloned from pTA231 was inserted into the middle of the flanking fragmentsThis study
E. coli strains
    DH5αF ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK mK) phoA supE44 thi-1 gyrA96 relA1Invitrogen
    DL739MC4100 recA dam-13::Tn939
H. volcanii strains
        H53ΔpyrE2 ΔtrpA24
        AF103H53 ΔartA14
        AF109AF103 containing pAF914
        ΔaglB mutantH53 ΔaglB31
        FH37H53 ΔpyrE2This study
        FH38H53 slgG796F,F797G ΔpyrE2This study