Relative phosphorylating activities of His-RhaB from B. subtilis in the presence of various factorsa

Reaction no.His-RhaBHis-RhaARhamnoseRhamnuloseATPMean relative activity (%) ± SDb
I+++100.0 ± 10.2
II+++0.275 ± 0.389
III++++29.2 ± 5.7
  • a Each reaction was carried out at 30°C for 30 min in the presence (+) or absence (–) of 10 μg of His-RhaB, 1 μg of His-RhaA, 10 mM rhamnose, 10 mM rhamnulose, and 1 mM ATP in a total volume of 500 μl, and then an aliquot of the reaction mixture was taken for quantification of ADP generated as a by-product of phosphorylation as described in Materials and Methods.

  • b The relative activities were based on the mean value of reaction mixture I (mean value ± the standard deviation in two independent experiments). ND, not detected.