TABLE 1

E. coli strains used in this study

Strain or phageGenotype or parent strain and descriptionaSource
Strains
    MG1655rph-1 (wt)E. Top, University of Idaho collection
    JW0604-1F Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) ΔcitT750::kan λ rph-1 Δ(rhaD-rhaB)568 hsdR514Keio collection, Yale University
    JW3496-1F Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ ΔdctA783::kan rph-1 Δ(rhaD-rhaB)568 hsdR514Keio collection, Yale University
    JW5437-1F Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ ΔrpoS746::kan rph-1 Δ(rhaD-rhaB)568 hsdR514Keio collection, Yale University
    B(wt) FColi Genetic Stock Center, Yale University
    REL606F tsx-467(Am) araA230 lon rpsL227 (Strr) hsdR [mal+]K-12S)Coli Genetic Stock Center, Yale University
    Cit+ mutants
        Mutants obtained by direct selection in M9C
            DV159MG1655 (wt), Cit+ at 30 days
            DV160MG1655 (wt), Cit+ at <40 daysThis study
            DV133TMG1655 (wt), phage transduction from DV133
            SO191JW3496-1 (ΔdctA), Cit+ at 134 daysThis study
            DV130JW5437-1 (ΔrpoS), Cit+ at 43 days
            DV133JW5437-1 (ΔrpoS), refined from DV130 via several cycles on SC agarThis study
            DV162JW5437-1 (ΔrpoS), Cit+ at <40 daysThis study
            DV268JW5437-1 (ΔrpoS), Cit+ at <40 daysThis study
        Mutants obtained by modified direct selection in M9C25 (glucose supported)
            DV247MG1655 (wt), Cit+ at 31 daysThis study
            DV290MG1655 (wt), Cit+ at 35 daysThis study
            DV291MG1655 (wt), Cit+ at 35 daysThis study
            DV309MG1655 (wt), Cit+ at 42 daysThis study
            DV312MG1655 (wt), Cit+ at 42 daysThis study
            DV313MG1655 (wt), Cit+ at 42 daysThis study
            DV314MG1655 (wt), Cit+ at 42 daysThis study
            DV315MG1655 (wt), Cit+ at 42 daysThis study
            DV409MG1655 (wt), Cit+ at 77 daysThis study
            DV344JW5437-1 (ΔrpoS), Cit+ at 68 daysThis study
        Mutants obtained by modified direct selection in M9CG50 (glycerol supported)
            DV179MG1655 (wt), Cit+ at 28 daysThis study
            DV215MG1655 (wt), Cit+ at 21 daysThis study
            DV172JW5437-1 (ΔrpoS), Cit+ at 19 daysThis study
            DV216JW5437-1 (ΔrpoS), Cit+ at 21 daysThis study
            DV351JW5437-1 (ΔrpoS), Cit+ at 35 days and refined from papillated colonyThis study
        Mutants obtained by modified direct selection in M9LC25 (glucose supported, 1.7 mM citrate [low citrate])
            DV564MG1655 (wt), Cit+ at 40 daysThis study
            DV596MG1655 (wt), Cit+ at 56 daysThis study
            DV600MG1655 (wt), Cit+ at 56 daysThis study
            DV592MG1655 (wt), Cit+ at 63 daysThis study
            DV593MG1655 (wt), Cit+ at 63 daysThis study
            DV594MG1655 (wt), Cit+ at 63 daysThis study
            DV595MG1655 (wt), Cit+ at 63 daysThis study
            DV599MG1655 (wt), Cit+ at 63 daysThis study
            DV601MG1655 (wt), Cit+ at 63 daysThis study
            DV602MG1655 (wt), Cit+ at 63 daysThis study
            DV603MG1655 (wt), Cit+ at 63 daysThis study
            DV604REL606, Cit+ at 63 daysThis study
        Mutants obtained by modified direct selection in M9LCG50 (glycerol supported, 1.7 mM citrate [low citrate])
            DV545MG1655 (wt), Cit+ at 35 daysThis study
            DV546MG1655 (wt), Cit+ at 35 daysThis study
            DV571MG1655 (wt), Cit+ at 40 daysThis study
            DV572MG1655 (wt), Cit+ at 41 daysThis study
            DV605B (wt), Cit+ at 63 daysThis study
            DV608B (wt), Cit+ at 63 daysThis study
            DV609B (wt), Cit+ at 63 daysThis study
            DV610B (wt), Cit+ at 63 daysThis study
            DV611REL606, Cit+ at 63 daysThis study
            DV613REL606, Cit+ at 63 daysThis study
            DV614REL606, Cit+ at 63 daysThis study
            DV615REL606, Cit+ at 63 daysThis study
            DV616REL606, Cit+ at 63 daysThis study
Phage P1(vir)Nonlysogenic transducing phageP. Hartzell
  • a wt, wild type; SC agar, Simmons citrate agar.