TABLE 1

Bacterial strains and plasmids utilized in this study

Strain or plasmidRelevant genotype or descriptionReference
Strains
    MB10DHB4 F′ lac pro lacIq Δ(malF)3 Δ(phoA)PvuII phoR Δ(lac)X174 Δ(ara-leu)7697 araD139 galE (or galU) galK62
    MB68MB10 ΔdsbA63
    MB3104MB10 dsbA::HaloThis study
    MB3105MB10 dsbA::HaloSSThis study
    MB3106MB10 dsbA::sfGFPThis study
    MB3769MB10 pDSW204-ssDsbA-HaloThis study
    MB3123MC410043
    MB4156MB3123 ΔrpoSThis study
    DHL708MC4100 ΔclpPX43
    MB3128MB3123 clpP::HaloThis study
    MB3717MB3123 clpP::msfGFPThis study
Plasmids
    pDSW204Mutant lacUV5 promoter, pBR322 origin, Ampr64
    pDSW204-ssDsbA-HaloSignal sequence of DsbA fused to the HaloTag in pDSW204This study
    pDHL584pUC19-linker-sfGFP-FRT-Kanr-FRT43
    pDHL940pUC19-linker-HaloTag H7-FRT-Kanr-FRTThis study
    pDHL1029pUC19-linker-msfGFP-FRT-Kanr-FRTThis study
    pUC57-HaloSS-KanrCysteineless HaloTag mutantThis study
    pDHL-HaloSSpUC19-linker-HaloSS-FRT-Kanr-FRTThis study