TABLE 3

Summary of fluorescence and bioluminescence visualizations of proteins in the two compartments of E. coli

ConstructReference for cytoplasmReference(s) for periplasm
GFP/sfGFP5520 (ssDsbA)
SNAP tag435658 (failed)
HaloTagThis workThis work (ssDsbA)
Luciferase59 (in Synechococcus)22 (ssOmpA)
Immunofluorescence6061