Table 1.

B. subtilis strains used in this study

StrainRelevant genotypeaSource, construction, or referenceb
168trpC2Laboratory collection
BBA0028PgntR-gfpmut3/specpBSBgntR → BSB168
BBA0118PfruR-gfpmut3/specpBSBfruR → BSB168
BBA0121PglpF-gfpmut3/specpBSBglpF → BSB168
BBA0223PbglP-gfpmut3/specpBSBbglP → BSB168
BBA0359PsacP-gfpmut3/specpBSBsacP → BSB168
BSB168Wild type5
GM1038sacA321 Δ(sacR-B)23 ccpA::Tn917Δ(erm lacZ)::phleoLaboratory collectionc
GM1222trpC2 pheA1 Δ(bgaX) amyE::(gntRK-lacZ) ptsH1∼cat11
GM2907ccpA::Tn917Δ(erm lacZ)::phleoGM1038 → BSB168
GM2924crh::aphA3QB7098 → BSB168
GM2933ptsH1∼catGM1222 → BSB168
GM3001ParaA-gfpmut3/specpBSBaraA → BSB168
GM3002PgntRcre2-gfpmut3/specpBSBgntRcre2 → BSB168
GM3003PsacPΔRAT-term-gfpmut3/specpBSBsacPΔRAT-term → BSB168
GM3004PglpFΔterm-gfpmut3/specpBSBglpFΔterm → BSB168
GM3005fruA-gfpmut3/specpBSBfruA → BSB168
GM3007PbglPΔRAT-term-gfpmut3/specpBSBbglPΔRAT-term → BSB168
GM3008ParaE-gfpmut3/specpBSBaraE → BSB168
GM3013ParaA-gfpmut3/spec ccpA::Tn917Δ(erm lacZ)::phleoGM3001 → GM2907
GM3014PgntRcre2-gfpmut3/spec ccpA::Tn917Δ(erm lacZ)::phleoGM3002 → GM2907
GM3015PsacPΔRAT-term-gfpmut3 ccpA::Tn917Δ(erm lacZ)::phleoGM3003 → GM2907
GM3016PglpFΔterm-gfpmut3/spec ccpA::Tn917Δ(erm lacZ)::phleoGM3004 → GM2907
GM3017fruA-gfpmut3/spec ccpA::Tn917Δ(erm lacZ)::phleoGM3005 → GM2907
GM3019PbglPΔRAT-term-gfpmut3/spec ccpA::Tn917Δ(erm lacZ)::phleoGM3007 → GM2907
GM3020ParaE-gfpmut3/spec ccpA::Tn917Δ(erm lacZ)::phleoGM3008 → GM2907
GM3022PglpF-gfpmut3/spec ccpA::Tn917Δ(erm lacZ)::phleoBBA0121 → GM2907
GM3023PbglP-gfpmut3/spec ccpA::Tn917Δ(erm lacZ)::phleoBBA0223 → GM2907
GM3024PsacP-gfpmut3/spec ccpA::Tn917Δ(erm lacZ)::phleoBBA0359 → GM2907
GM3027PfruR-gfpmut3/spec ccpA::Tn917Δ(erm lacZ)::phleoBBA0118 → GM2907
GM3029PgntRcre2-gfpmut3/spec crh::aphA3GM3002 → GM2924
GM3031PbglP-gfpmut3/spec crh::aphA3BBA0223 → GM2924
GM3033PbglPΔRAT-term-gfpmut3/spec crh::aphA3GM3007 → GM2924
GM3037PgntRcre2-gfpmut3/spec ptsH1∼catGM3002 → GM2933
GM3039PbglP-gfpmut3/spec ptsH1∼catBBA0223 → GM2933
GM3043PbglPΔRAT-term-gfpmut3/spec ptsH1∼catGM3007 → GM2933
GM3059fruA-gfpmut3/spec crh::aphA3GM3005 → GM2924
GM3057PglpFΔterm-gfpmut3/spec crh::aphA3GM3004 → GM2924
GM3061PfruR-gfpmut3/spec crh::aphA3BBA0118 → GM2924
GM3063ParaE-gfpmut3/spec crh::aphA3GM3008 → GM2924
GM3065ParaA-gfpmut3/spec crh::aphA3GM3001 → GM2924
GM3067PsacPΔRAT-term-gfpmut3 crh::aphA3GM3003 → GM2924
GM3069PglpFΔterm-gfpmut3/spec ptsH1∼catGM3004 → GM2933
GM3071fruA-gfpmut3/spec ptsH1∼catGM3005 → GM2933
GM3073PfruR-gfpmut3/spec ptsH1∼catBBA0118 → GM2933
GM3075ParaE-gfpmut3/spec ptsH1∼catGM3008 → GM2933
GM3077ParaA-gfpmut3/spec ptsH1∼catGM3001 → GM2933
GM3079PsacPΔRAT-term-gfpmut3 ptsH1∼catGM3003 → GM2933
GP270trpC2 xylR::ermC51
GP284trpC2 xylR::ermC ptsH151
GP287trpC2 xylR::ermC ptsH1 crh::spc51
GP289trpC2 xylR::ermC hprK::aphA351
GP297trpC2 xylR::ermC crh::spc51
GP853trpC2 xylR::ermC ccpA::spc51
GP1303trpC2 ccpA-Strep spcpGP1952 → 168
QB7098trpC2 crh::aphA3 amyE::(PΔB levD′-′lacZ cat)17
  • a PgntRcre2-gfpmut3 corresponds to a fusion with both cre sites, including cre2 internal to the gntR ORF; PsacPΔRAT-term-gfpmut3, PbglPΔRAT-term-gfpmut3, and PglpFΔterm-gfpmut3 correspond to fusions without the regulatory region (terminator and RAT sequence, when relevant) and are therefore expressed constitutively.

  • b Arrows indicate construction by transformation.

  • c GM1038 was derived in several steps from strain TH256, a ccpA mutant by Tn917lac transposition obtained by Tina Henkin (57).