Table 4.

Repression by malate or glucose in the ptsH1 and Δcrh single mutants

FusionptsH1 genetic contextΔcrh genetic context
StrainExpression levelaStrainExpression levela
+ Glucose+ Malate+ Glucose+ Malate
PbglPΔRAT-term-gfpGM30432627GM3033830
PsacPΔRAT-term-gfpGM30792035GM3067432
PgntRcre2-gfpGM30372765GM3029955
PglpFΔterm-gfpGM30694550GM30572040
fruA-gfpGM30717282GM30595584
PfruR-gfpGM30738090GM3061>90b87
ParaE-gfpGM3075269GM30631420
ParaA-gfpGM30773943GM3065519
  • a Expression level is given as a percentage of the expression in the presence of the suitable inducer for fusions requiring induction and of the expression in M9SE for constitutive fusions. Mean values of three independent experiments with standard deviations of <±10% are presented.

  • b Expression level comprised between 90 and 100% and was not distinguishable from the induced condition with this assay.