TABLE 1.

Bacterial strains and plasmids

Strain or plasmidGenotypeResistanceaSource
Escherichia coli strains
    CV1Chemotaxis wild type (same as RP437)Str24
    TG1/pDS-Red ExpressWild type; dead-cell controlAmpStratagene
    CV5CV1 ΔtsrStrThis studyb
    CV12CV1 Δtar-tap trg::Tn10Str, TetThis studyc
    MJ101CV1 lsrBΩKanrStr, KanThis studyd
    MJ102CV1 lsrCΩKanrStr, KanThis studye
    BW25113 ΔlsrBlsrBΩKanrKan3
    BW25113 ΔlsrClsrCΩKanrKan3
Plasmids
    pCM18GFP-expressing vectorErm12
    pDS-RedExpressRFP-expressing vectorAmpClontech
    pCA24N-lsrBpCA24N PT5-lac::lsrB; expresses E. coli lsrB from placZYACm15
  • a Str, streptomycin; Tet, tetracycline; Kan, kanamycin; Erm, erythromycin; Amp, ampicillin; Cm, chloramphenicol.

  • b Made by introducing Δtsr9101 (7) into CV1 by phage P1 transduction (16), with selection for Thr+ and screening for Tsr.

  • c Made in two steps: Δtar-tap5201 (26) was introduced into CV1 by phage P1 transduction with selection for Eda+, followed by screening for Tar, and then trg::Tn10 was introduced by phage P1 transduction followed by selection for Tetr on lysis broth (LB) (20) agar plates containing 10 μg/ml tetracycline, followed by screening for Trg.

  • d Made by introducing lsrBΩKanr (3) into CV1 by phage P1 transduction, with selection for Kanr and confirmation of lsrB gene disruption by PCR.

  • e Made by introducing lsrCΩKanr (3) into CV1 by phage P1 transduction, with selection for Kanr and confirmation of lsrC gene disruption by PCR.