TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsaSource or reference
Strains
    AM604MC4100 ara+41
    AM661AM604 ΔlptD::kan/araBp-lptD29
    AM689AM604 ΔlptE::kan Δ(λatt-lom)::bla araBp-lptE41
    DH5αΔ(argF-lac169) 80dlacZ58(M15) glnV44(AS) λ rfbD1 gyrA96 recA1 endA1 spoT1 thi-1 hsdR1711
    FL905AM604 Φ(kan araC araBp-lptC)129
    FL907AM604 Φ(kan araC araBp-lptA)129
    M15/pREP4F lac thi mtl/pREP4Qiagen
Plasmids
    pRSETT7 promoter; AprInvitrogen
    pET30bT7 promoter; KmrNovagen
    pET30-lptC-lk25-HpET30b derivative, expresses a LptC-lk25-His6 version where lk is a 25-amino-acid linker region separating LptC residue 191 from the His6 affinity tagThis study
    pQE30T5 promoter; AprQiagen
    pQEsH-lptCpQE30 derivative, expresses His6-LptC24-191This study
    pGS100pGZ119EH derivative, contains TIR sequence downstream of Ptac; Cmr30
    pGS103pGS100 Ptac-lptC30
    pGS103Δ177-191pGS103 Ptac-lptCΔ177-191This study
    pGS103G56VpGS103 Ptac-lptC(G56V)This study
    pGS103Y112S-G153RpGS103 Ptac-lptC(Y112S-G153R)This study
    pGS103G153RpGS103 Ptac-lptC(G153R)This study
    pGS108pGS100 Ptac-lptC-H30
    pGS108Δ177-191pGS108 Ptac-lptCΔ177-191-HThis study
    pGS108G153RpGS108 Ptac-lptC(G153R)-HThis study
    pGS108G56VpGS108 Ptac-lptC(G56V)-HThis study
    pGS116pGS103Δ177-191 derivative expressing both the truncated LptCΔ177-191 and LptC-lk25 from pET30- lptC-lk25-H plasmidThis study
  • a Cmr, chloramphenicol resistance; Apr, ampicillin resistance; Kmr, kanamycin resistance.