TABLE 2.

Bacterial strains, genomic DNA, and plasmids used in this study

Strain, genomic DNA source, or plasmidRelevant characteristic(s)aReference
Strains
    E. coli
        DH5αλ φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK mK) supE44 thi-1 gyrA relA156
        S17I-λpirrecA pro thi hsdRM+ RP4-2 Tc::Mu-Km::Tn7-λpir12
        W3110F IN(rrnD-rrnE)11
        ΔftsH strainW3110 zad220::Tn10 sfhC21 ΔftsH3::kan60
        RH166MC4100 Δara Δleu lac mutant6
        Δlon strainRH166 Δlon::Tn105
        BL21(DE3)F ompT hsdSB(rB mB−) gal dcm (DE3)57
        AR5088BL21(DE3) zad220::Tn10 sfhC21 ΔftsH3::kan33
    S. enterica serovar Typhimurium M556SL1344 sseD::aphT26
    P. aeruginosa PAO1Wild type13
Origins of genomic DNA
    E. coli K12Wild type1
    S. enterica serovar Typhimurium M556SL1344 sseD::aphT26
    P. aeruginosa PAO1Wild type13
    A. aolicus VF5Wild type11
    R. capsulatus B10SWild type37
    A. tumefaciens C58Wild type61
    V. cholerae O1 serovar El Tor strain N16961Wild type27
    Y. pseudotuberculosis YPIIIInv+ (pIB1)18
Plasmids
    pBAD24Ampr; PBAD araC25
    pBO110pBAD24 derivative coding for LpxCEc15
    pBO197pBAD24 derivative coding for N-terminal His6 tag fusionsH. Baumann
    pET19bAmpr; PT7; codes for N-terminal His10 tag fusionsNovagen
    pASK-IBA5(+)Ampr; P/Otet tetR; codes for N-terminal Strep tag fusionsIBA GmbH
    pBO1721pASK-IBA5(+) derivative; the coding region for the Strep tag was replaced by a His6 sequenceS. Hagen
    pHERD20TAmpr; PBAD araC; E. coli-P. aeruginosa shuttle vector52
    pBO1702pET19b derivative coding for His10-LpxCEcThis study
    pBO2382pASK-IBA5(+) derivative coding for His10-LpxCEcThis study
    pBO2381pASK-IBA5(+) derivative coding for His10-LpxCAaThis study
    pBO1718pASK-IBA5(+) derivative coding for His6-LpxCRcThis study
    pBO1144pBAD24 derivative coding for His6-LpxCAtThis study
    pBO1187pBAD24 derivative coding for His6-LpxCAtΔC13This study
    pBO1177pBAD24 derivative coding for His6-LpxCPaThis study
    pBO1146pBAD24 derivative coding for His6-LpxCVcThis study
    pBO1173pET19b derivative coding for His10-LpxCYpThis study
    pBO1142pBAD24 derivative coding for LpxCSeThis study
    pBO1172pBAD24 derivative coding for LpxCSeΔC5This study
    pBO1745pHERD20T derivative coding for His6-LpxCPaThis study
  • a Ampr, ampicillin resistance. For truncated proteins, the number of C-terminally deleted residues is indicated (ΔC).