Table 1.

Bacterial strains and plasmids used in this study

Strain, genotype, or plasmidDescriptionSource or reference
C. rodentium strain or genotype
    DBS100Wild-type C. rodentium strain (ATCC 51459)32
    ΔphoPQDBS100 ΔphoPQ21
    ΔpmrABDBS100 ΔpmrABThis study
    ΔpmrCDBS100 ΔpmrCThis study
    ΔcptADBS100 ΔcptAThis study
    ΔpmrC ΔcptADBS100 ΔpmrC ΔcptAThis study
    ΔphoPQ ΔpmrABDBS100 ΔphoPQ ΔpmrABThis study
    DBS100 pmrC::lacZpmrC::lacZ in DBS100, CmrThis study
    ΔphoPQ pmrC::lacZpmrC::lacZ in ΔphoPQ, CmrThis study
    ΔpmrAB pmrC::lacZpmrC::lacZ in ΔpmrAB, CmrThis study
    DBS100 cptA::lacZcptA::lacZ in DBS100, CmrThis study
    ΔphoPQ cptA::lacZcptA::lacZ in ΔphoPQ, CmrThis study
    ΔpmrAB cptA::lacZcptA::lacZ in ΔpmrAB, CmrThis study
E. coli strain
    χ7213thr-1 leuB6 fhuA21 lacY1 glnV44 recA1 asdA4 thi-1 RP4-2-Tc::Mu [-pir] Kanr30
Plasmids
    pRE112Sucrose-sensitive (sacB1) suicide vector, Cmr9
    pFUSESuicide vector, lacZYA, mob+ (RP4), ori R6K, Cmr2
    pWSK129Low-copy-no. cloning vector, Kanr38
    pWSKpmrABThis study
    pWSKpmrCThis study
    pWSKcptAThis study