Table 2.

Primers used in this study

PrimerSequenceaUsage
CR677AGCTAGTCTAGATGTTCTCCGGAATGCCACGCAAGCΔpmrAB 5′F XbaI
CR678AGCTAGGATATCGGTGTCGCGGGCGGTCAGGATCAGΔpmrAB 5′R EcoRV
CR679AGCTAGGATATCGCGGTGATGGGAGTTGAGGATCAGΔpmrAB 3′F EcoRV
CR680AGCTAGGAGCTCTTTCGCCGGTCTGCTGATGCTGGCΔpmrAB 3′R SacI
CR694GACTAGTCTAGACCAGCAGAATCAGGCCGATAGΔpmrC 5′F XbaI
CR695AGCCATGATATCACGGCGTAAATGCGGACTCGΔpmrC 5′R EcoRV
CR696AGCTAGGATATCCAGCGGCATCGACAGAAAGACΔpmrC 3′F EcoRV
CR697CAGTGAGAGCTCCCGCTACATTCCAGAAGCCCTΔpmrC 3′R SacI
CR719TGACATCTAGAGGAGCTGGGCAAACTACCGCTCΔcptA 5′F XbaI
CR734TGCAGGTACCGCTAAATGCGGACGCGGGCTGGΔcptA 5′R KpnI
CR735GGTGGGTACCTGCGCTGATTGATTATGACACTCTGΔcptA 3′F KpnI
CR722GGCGGAGCTCTCAGGTCGTAGCCCTTCAGCAACΔcptA 3′R SacI
CR555AGCTAGTCTAGAGATCTTAGCCCAGCTTTCGCAGTATCCGGTCTCCpmrC::lacZ 5′F XbaI
CR554AGCTACCCCGGGAAACCAGCAGCAGCCAGGCGAGCAGTCTGAGpmrC::lacZ 3′R SmaI
CR774AGCTAGTCTAGAATGCTGGAAATGGTCTTCGCCAAAGcptA::lacZ 5′F XbaI
CR775AGCTACCCCGGGGCTAAATGCGGACGCGGGCTGGcptA::lacZ 3′R SmaI
CR756GGTATCTACAGAAATGGTCTTCGCCAAAGCGGATpWSKcptA 5′ XbaI
CR757GCCAGAGCTCGATGTCCTCAAGTGATTATTGCCGGpWSKcptA 3′ SacI
  • a Restriction sites are underlined.