Table 1

Strains and plasmids used in this work

Strain or plasmidRelevant characteristicsaSource or reference
Strains
    E. coli DH5αλ ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK mK) supE44 thi-1 gyrA relA1Invitrogen, America
    E. coli BL21(DE3)F ompT hsdSB(rB mB) gal dcm (DE3)Invitrogen, America
    P. aeruginosa XMGWild-type strain capable of lactate utilizationThis study
    P. aeruginosa CMiLDHs constitutive mutant of P. aeruginosa XMGThis study
Plasmids
    pEasy-BluntCloning vector; Apr and KmrTransgene
    pEasy-Blunt-lldRpEASY-Blunt with lldR gene of P. aeruginosa XMGThis study
    pEasy-Blunt-lldRMpEASY-Blunt with lldR gene of P. aeruginosa CMThis study
    pETDuet-1Vector for protein expression; AprNovagen
    pETDuet-lldRpETDuet-1 with lldR gene of P. aeruginosa XMGThis study
    pETDuet-lldRMpETDuet-1 with lldR gene of P. aeruginosa CMThis study
  • a Kmr and Apr indicate resistance to kanamycin and ampicillin, respectively.