Table 1

Strains and plasmids used in this study

Strain or plasmidGenotype or relevant characteristicsaReference or source
Plasmids
    pBBR1MCS3Cloning vector (Tc)16
    pRK415Cloning vector (Tc)14
    pBBR-cheA1pBBR1MCS3 containing cheA14
    pBBR-cheA1H252QpBBR1MCS3 containing cheA1H252QThis work
    pBBR-cheB1pBBR1MCS3 containing cheB1This work
    pBBR-cheB1D78NpBBR1MCS3 containing cheB1D78NThis work
    pRK-cheY1pRK415 containing cheY14
    pRK-cheY1D52NpRK415 containing cheY1D52NThis work
Strains
    Escherichia coli
        TOP10General cloning strainInvitrogen
        S17-1thi endA recA hsdR with RP4-2Tc::Mu-Km::Tn7 integrated in chromosome26
    Azospirillum brasilense
        Sp7Parental strain, wild typeATCC 29145
        AB101Δ(cheA1)::gusA-Km (Km)4
        GA3Δ(cheB1)::gusA-Km (Km)29
        BS109Δ(cheR1)::Gm (Gm)29
        BS104Δ(cheB1 cheR1)::Km (Km)29
        AB102Δ(cheY1)::Km (Km)4
        AB103 (Δche1 mutant)Δ(cheA1 cheR1)::Cm (Cm)4
        AB301ΔaerC::Km (Km)37
        AB302 (Δche1 ΔaerC mutant)Δ(cheA1 cheR1) Δ(aerC)::Km (Cm Km)This work
        Sp72002flcA::Tn5 (Km)21
        AB104Δ(cheA1 cheR1) flcA::Tn5 (Cm Km)This work
        Wild type(pRK415)Wild-type strain (Sp7) containing pRK415 (Tc)4
        Wild type(pBBR)Wild-type strain (Sp7) containing pBBR1MCS3 (Tc)4
        AB101(pBBR)AB101 containing pBBR1MCS3 (Tc)4
        AB101(pBBR-cheA1)AB101 containing pBBR-cheA1 (Tc)4
        AB101(pBBR-cheA1H252Q)AB101 containing pBBR-cheA1H252Q (Tc)This work
        GA3(pBBR)GA3 containing pBBRThis work
        GA3(pBBR-cheB1)GA3 containing pBBR-cheB1This work
        GA3(pBBR-cheB1D78N)GA3 containing pBBR-cheB1D78NThis work
        AB102(pRK415)AB102 containing pRK4154
        AB102(pRK-cheY1)AB102 containing pRK-cheY14
        AB102(pRK-cheY1D52N)AB102 containing pRK-cheY1D52NThis work
  • a Antibiotic resistance abbreviations: Tc, tetracycline; Km, kanamycin; Gm, gentamicin; Cm, chloramphenicol.