Table 2

Time course of clumping and flocculation in wild-type and mutant derivatives of A. brasilense

StrainTime (h)a to:
Initiation of transient clumpingbTransition to stable clumpingcFlocculation
Sp79 ± 111 ± 124 ± 3
AB101 (ΔcheA1)2 ± 13 ± 217 ± 1
GA3 (ΔcheB1)2 ± 13 ± 211 ± 1
AB102 (ΔcheY1)6 ± 29 ± 114 ± 1
AB103 (Δche1)9 ± 111 ± 119 ± 2
BS109 (ΔcheR1)9 ± 111 ± 124 ± 3
BS104 [Δ(cheB1 cheR1)]
Sp72002 (flcA::Tn5)9 ± 111 ± 1
AB104 (Δche1 flcA::Tn5)9 ± 113 ± 1
AB301 (ΔaerC)2 ± 13 ± 214 ± 1
AB302 (ΔaerC Δche1)9 ± 111 ± 119 ± 2
  • a Values represent the time (mean ± standard deviation) postinoculation into flocculation media (n = 5). —, no clumping or no flocculation.

  • b Time at which transient cell-to-cell clumping is first detected. The fraction of transient clumps increases over time.

  • c Time at which stable cell-to-cell clumping is first detected. The fraction of stable clumps increases over time.