Table 2

Kinetic constants of various cyanuric acid hydrolase/barbiturase family enzymes, using cyanuric acid as a substrate

Enzyme name and /or organism (reference)kcat (s−1)Km (μM)kcat/Km (s−1 M−1)
TrzD (20)250505 × 106
TrzD (this study)14.2 ± 0.458 ± 7(2.5 ± 0.4) × 105
Pseudomonas sp. ADP AtzD (12)6.8 ± 0.757 ± 10(1.2 ± 0.3) × 105
Pseudomonas sp. ADP AtzD (this study)73 ± 623 ± 7(3.2 ± 1.2) × 106
Moorella thermoacetica ATCC 39073 (23)10.61101.0 × 105
Bradyrhizobium japonicum USDA 1109.3 ± 0.750 ± 10(1.9 ± 0.5) × 105
Rhizobium leguminosarum bv. viciae 38415 ± 1130 ± 60(3.8 ± 2.5) × 104
Methylobacterium sp. 4-4617 ± 269 ± 16(2.5 ± 0.9) × 105
Azorhizobium caulinodans ORS 571 locus AZC_389250 ± 9370 ± 90(1.3 ± 0.6) × 105
Azorhizobium caulinodans ORS 571 locus AZC_3203a
  • a —, no activity with any substrate tested.