Table 1

Bacterial strains and plasmids used in this study

Bacterial strain or plasmidRelevant featureSource or reference
Strains
    M. xanthus
        DK1622Wild type13a
        DK4398asgB19a
        AG1400DK1622 Δnla28SThis study
        AG1401Δnla28S + nla28S (pZS202)This study
        AG1402Δnla28S + nla28S H242A (pZS210)This study
    E. coli
        TOP10Cloning hostInvitrogen
        BL21(DE3)Expression hostLab collection
        NiCO21(DE3)Expression hostNew England Biolabs
Plasmids
    pCR-BluntCloning vector, KanrInvitrogen
    pGEX-4T3GST fusion expression vector, AmprGE Healthcare
    pET28bHis tag fusion expression vector, KanrEMD Biosciences
    pBJ114Plasmid used for gene replacements and deletions, Kanr galK+47
    pZS93bnla28S deletion cassette in pCR-BluntThis study
    pZS93nla28S deletion cassette in pBJ114This study
    pZS108bnla28S-cyt in pCR-BluntThis study
    pZS109bnla28 in pCR-BluntThis study
    pZS108nla28S-cyt in pGEX-4T3This study
    pZS109nla28 in pGEX-4T3This study
    pZS134benvZ-cyt in pCR-BluntThis study
    pZS135bompR in pCR-BluntThis study
    pZS134envZ-cyt in pET28bThis study
    pZS135ompR in pET28bThis study
    pZS187nla28S-cyt H242A in pGEX-4T3This study
    pZS188nla28S-cyt D386A in pGEX-4T3This study
    pZS191nla28- D53A in pGEX-4T3This study
    pZS2021,000-bp upstream region + nla28S in pCR-BluntThis study
    pZS2101,000-bp upstream region + nla28S H242A in pCR-Blunt