Table 1

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype and/or descriptionaSource or reference
Plasmids
    pIJ8660Promoter probe vector containing the egfp gene as indicator; oriT RK2 attP aac(3)IV20
    pIJ8660-hrdBp896-bp PCR fragment of hrdB promoter region inserted into the KpnI-BamHI site of pIJ866016
    pIJ8660-amtBpWT500-bp PCR fragment of the wild-type amtB promoter region inserted into the BamHI-NdeI site of PIJ8660This study
    pIJ8660-amtBp3a3-b3 site mutation within the amtB promoter region from PIJ8660-amtBpWTThis study
    pIJ8660-amtBp1a1-b1 site mutation within the amtB promoter region from PIJ8660-amtBpWTThis study
    pIJ8660-amtBp2a2-b2 site mutation within the amtB promoter region from PIJ8660-amtBpWTThis study
    pET28aExpression vector carrying N-terminal His tag; KmrNovagen
    pEXSCRpET28a carrying the S. coelicolor glnR gene23
    pET32aExpression vector carrying the N-terminal Trx-His-S tag; AmprNovagen
    pET32a-PhoPpET32a carrying the S. coelicolor phoP geneThis study
Strains (S. coelicolor)
    M145Wild type, SCP1 SCP2John Innes Centre
    M145/pIJ8660M145 integrated with pIJ8660This study
    SCamtB-WTM145 integrated with pIJ8660-amtBpWTThis study
    SCamtB-3M145 integrated with pIJ8660-amtBp3This study
    SCamtB-1M145 integrated with pIJ8660-amtBp1This study
    SCamtB-2M145 integrated with pIJ8660-amtBp2This study
    M145/pIJ8660-hrdBpM145 integrated with pIJ8660-hrdBp17
  • a Ampr, ampicillin resistance; Kmr, kanamycin resistance.