Table 1

Genes and proteins of the CRP-S regulon of H. influenzaea

GeneLocus tagReported H. influenzae mutant phenotype (reference[s])H. influenzae protein sequence predictioncHomolog(s)Function(s) of homolog(s) (reference[s])
pilAHI0299Uptake, TF (32, 104)16 kDa, pI 9.3; prepilin peptidase signal; periplasmic and extracellular locationspilE (Neisseria), pilA (Pseudomonas)Major pilin subunit (2, 58, 73, 77)
pilBHI0298Uptake, TF (32)53 kDa, pI 5.7; no signal detected; cytoplasmic locationpilB (Neisseria, Pseudomonas)ATP-dependent extension of pilus subunits (39, 74)
pilCHI0297Uptake, TF (32)47 kDa, pI 9.4; no signal detected; IM location, 3 transmembrane helicespilC (Neisseria, Pseudomonas)Pilus assembly (39, 74, 102)
pilDHI0296None available27 kDa, pI 9.3; no signal detected; IM location, 7 transmembrane helicespilD (Neisseria, Pseudomonas)Prepilin peptidase (25, 39, 74, 75)
comAHI0439Uptake, TF (101)32 kDa, pI 5.7; no signal detected; IM locationpilM (Neisseria, Pseudomonas)Pilus assembly (7, 18, 65)
comBHI0438None available20 kDa, pI 8.3; noncleavable signal peptide; location unknownpilN (Neisseria, Pseudomonas)Pilus assembly (7, 18, 65, 86, 98)
comCHI0437Uptake, TF (101)20 kDa, pI 9.3; signal peptidase I signal; location unknownpilO (Neisseria, Pseudomonas)Pilus assembly (7, 18, 65, 86, 98)
comDHI0436None available16 kDa, pI 4.6; signal peptidase II signal; location unknownpilP (Neisseria, Pseudomonas)Pilus assembly, IM lipoprotein; interacts with secretin (7, 8, 18, 65, 98)
comEHI0435Uptake, TF (32, 101)49 kDa, pI 7.4; signal peptidase I signal; OM locationpilQ (Neisseria, Pseudomonas)OM secretin, binds T4P and DNA (5, 28, 65)
comFHI0434WT uptake, TF (100, 101)27 kDa, pI 10.3; no signal detected; cytoplasmic locationcomF (Neisseria, Pseudomonas)Function unknown
comNHI0938None available20 kDa, pI 9.6; prepilin peptidase signal; location unknownNo homolog foundFunction unknown
comOHI0939TF (107)27 kDa, pI 9.6; signal peptidase I signal; location unknownNo homolog foundFunction unknown
comPHI0940None available25 kDa, pI 9; signal peptidase I signal; location unknownNo homolog foundFunction unknown
comQHI0941None available12 kDa, pI 9.9; signal peptidase I signal; location unknownNo homolog foundFunction unknown
comE1HI008None available12 kDa, pI 8.3; signal peptidase I signal; location unknowncomE (Neisseria, Pseudomonas), comEA (Bacillus)DNA binding protein (23, 45, 72, 82)
HI0365HI0365WT uptake and TF (107)44 kDa, pI 6.7; no signal detected; cytoplasmic location; rRNA large subunit methyltransferase N; Fe-S cluster redox enzymeHypothetical protein (Neisseria, Pseudomonas)Function unknown
pilF2HI0366Uptake, TF (107)21 kDa, pI 8.9; signal peptidase I signal; location unknown; tetratricopeptide repeatspilW (Neisseria), pilF (Pseudomonas)Pilotin, stabilizes secretin multimers (3, 19, 50, 51, 95, 105)
rec2HI0061WT uptake, TF (68, 90)90 kDa, pI 9.7; no signal detected; IM location, 13 transmembrane helicescomA (Neisseria), rec2 (Pseudomonas), comEC (Bacillus)Putative DNA membrane channel (34)
ligAHI1182, HI1183bWT uptake, TF down (107)31 kDa, pI 9.2; signal peptidase I signal; location unknownadl (Neisseria)Periplasmic ATP-dependent DNA ligase (63)
HI0659HI0659None available11 kDa, pI 9.2; no signal detected; cytoplasmic location; HTH motifNo homolog foundFunction unknown
HI0660HI0660None available14 kDa, pI 9.46; no signal detected; cytoplasmic location; putative Holliday junction resolvaseNo homolog foundFunction unknown
HI1631HI1631None available22 kDa, pI 9.5; no signal detected; location unknown; restriction endonuclease-like superfamily; putative Holliday junction resolvaseNo homolog foundFunction unknown
comMHI1117WT uptake, TF down (42)56 kDa, pI 9.2; no signal detected; cytoplasmic location; putative Mg chelatase subunitcomM (Neisseria, Pseudomonas)Function unknown
dprAHI0985TF (48, 100)42 kDa, pI 6.3; no signal detected; cytoplasmic locationdprA (Neisseria), smf (Pseudomonas)Protects DNA from degradation in cytoplasm (4)
radCHI0952None available25 kDa, pI 6.2; no signal detected; cytoplasmic locationradC (Pseudomonas)Replication fork stabilization and repair (6, 87)
ssbHI0250None available19 kDa, pI 5.3; no signal detected; cytoplasmic locationssb (Neisseria, Pseudomonas)Ubiquitous single-stranded-DNA binding protein (83)
  • a TF, transformation frequency; WT, wild type; IM, inner membrane; OM, outer membrane; T4P, type IV pilus.

  • b Incorrectly annotated as two open reading frames due to a translational frameshift.

  • c Predictions run with LipoP, PsortB, TargetP, SignalP, Pilfind, and InterProScan.