Table 1

Strains and plasmids

Strain or plasmidRelevant genotype, description, or sequenceReference or source
E. coli strains
    DH5αl f80dlacZDM15D(lacZYA-argF)U169 recA1 endA hsdR17(rK mK) supE44 thi-1 gyrA relA1Life Technologies
    S17-1 (λpir)thi pro hsdR- hsdM+ ΔrecA RP4-2::TcMu-Km::Tn755
P. aeruginosa strains
    PA14Wild type
    bifA strainUnmarked in-frame deletion of the bifA gene30
    bifA pilY1 strainUnmarked in-frame deletions of bifA and pilY129
    bifA pilA strainUnmarked in-frame deletions of bifA and pilA29
    bifA fimU strainUnmarked in-frame deletions of bifA and fimUThis study
    bifA pilV strainUnmarked in-frame deletions of bifA and pilVThis study
    bifA pilW strainUnmarked in-frame deletions of bifA and pilWThis study
    bifA pilX strainUnmarked in-frame deletions of bifA and pilXThis study
    bifA pilE strainUnmarked in-frame deletions of bifA and pilEThis study
    bifA fimU-pilVWXY1Y2E strainUnmarked deletions of bifA and the fimU-pilVWXY1Y2E operonThis study
    bifA pilD strainUnmarked in-frame deletions of bifA and pilDThis study
    pilX+::His strainPA14 with chromosomal insertion of a C-terminal His6 tag in the pilX geneThis study
    pilX(G−1F)::His strainPA14 with chromosomal insertion of a His6 tag and mutation of G−1 to F in the pilX geneThis study
    pilXΔLP::His strainPA14 with chromosomal deletion of the pilX leader peptide sequence and insertion of a His6 epitopeThis study
    bifA pilX+::His strainbifA mutant with chromosomal insertion of a C-terminal His6 tag in the pilX geneThis study
    bifA pilX(G−1F)::His strainbifA mutant with chromosomal insertion of a His6 tag and mutation of G−1 to F in the pilX geneThis study
    bifA pilXΔLP::His strainbifA mutant with chromosomal deletion of the pilX leader peptide sequence and insertion of a His6 epitopeThis study
    pilD pilX+::His strainpilD mutant with chromosomal insertion of a C-terminal His6 tag in the pilX geneThis study
    pilD pilXΔLP::His strainpilD mutant with chromosomal deletion of the pilX leader peptide sequence and insertion of a His6 epitopeThis study
Plasmids
    pMQ72Shuttle vector for cloning in yeast and for arabinose-inducible gene expression; Gmr52
    pMQ30Shuttle vector for yeast cloning and Gram-negative allelic replacement; Gmr52
    pMQ30-fimUPlasmid for in-frame deletion of fimU gene; GmrThis study
    pMQ30-pilVPlasmid for in-frame deletion of pilV gene; GmrThis study
    pMQ30-pilWPlasmid for in-frame deletion of pilW gene; GmrThis study
    pMQ30-pilXPlasmid for in-frame deletion of pilX gene; GmrThis study
    pMQ30-pilEPlasmid for in-frame deletion of pilE gene; GmrThis study
    pMQ30-pilDPlasmid for in-frame deletion of pilD gene; GmrThis study
    pMQ30-fimUpilVWXY1Y2EPlasmid for in-frame deletion of the fimU-pilVWXY1Y2E operon; GmrThis study
    pMQ30-pilX+::HisPlasmid for chromosomal insertion of a His6 tag in the pilX geneThis study
    pMQ30-pilX(G−1F)::HisPlasmid for chromosomal insertion of a His6 epitope and an amino acid substitution of G−1 to F in pilXThis study
    pMQ30-pilXΔLP::HisPlasmid for chromosomal deletion of the pilX leader peptide sequence and insertion of a His6 epitope
    pPilX-HisHis-tagged pilX gene in pMQ72; GmrThis study
    pPilX(G−1A)-HisHis-tagged pilX gene with the G−1A substitution cloned in pMQ72; GmrThis study
    pPilX(G−1D)-HisHis-tagged pilX with the G−1D substitution cloned in pMQ72; GmrThis study
    pPilX(G−1F)-HisHis-tagged pilX gene with the G−1F substitution cloned in pMQ72; GmrThis study
    pPilW-HisHis-tagged pilW gene in pMQ72; GmrThis study
    pPilW(G−1F)-HisHis-tagged pilW gene with the G−1F substitution cloned in pMQ72; GmrThis study
    pPilY1-HisHis-tagged pilY1 gene cloned in the pMQ80 expression vector29