Table 3

Amylase production by various mutant strains

StrainAmylase productiona
168 (wt)b1.99 ± 0.26
TI392 (uppS86)3.36 ± 0.42*
TI408 (PS10-uppS)2.48 ± 0.53
TI403 (yubB)2.88 ± 0.48*
TI409 (PS10-yubB)2.12 ± 0.32
TI393 (bcrC)2.73 ± 0.45*
  • a B. subtilis strains were grown in NG medium at 30°C. After 72 h of cultivation, the culture supernatant obtained after centrifugation was used for amylase assay. Amylase production is expressed as amylase activity (units) as described previously (12). The asterisks denote significant differences (P < 0.05).

  • b wt, wild type.