Table 1

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype and/or phenotypeSource or reference
V. cholerae
    N16961Wild type, lacking hapR function, O1 serogroup, biotype El Tor, Smr14
    C6709Wild type, hapR+, O1 serogroup, biotype El Tor, Smr20
    N-DksA1N16961 ΔdksAkan; Kmr SmrThis study
    C-DksA1C6709 ΔdksAkan; Kmr SmrThis study
    NRVDK2N-DksA1 ΔrelVaadA1; Kmr Smr SprThis study
E. coli
    DH5αF′ endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 Δ(argF-lacZYA)U169 (ϕ80dlacZΔM15)Promega
    SM10λpirthi thr leu tonA lacY supE recA∷RP4-2-Tc∷Mu λpir R6K20
    S17-1λpirthi proA hsdR recA∷RP4-2-Tc∷Mu-1 kan∷Tn7 integrant λpir R6K; Tpr SmrLab stock
    CF1648Wild type MG165554
    CF9240CF1648 ΔdksATet; Ttr9
Plasmids
    pDrivepUC origin, high-copy-no. cloning vector; Apr KmrQiagen
    PBluescript II KS(+)ColE1, high-copy-no. cloning vector; AprStratagene
    pKAS32rpsL suicide vector with oriR6K mobRP4; AprLab stock
    pBR322pMB1 origin, general-purpose cloning vector; Apr TtrLab stock
    pUC4KSource of kanamycin resistance gene cassette; Apr KmrPharmacia
    pBAD24pBR322 origin, l-arabinose-inducible vector; AprLab stock
    pBS202.9-kb ΔrelVaadA1 allele in pKAS32; Apr Spr14
    pJK5372.3-kb dksA region of E. coli strain MG1655 in pBR322; Apr9
    pDDKW1841-bp dksA region of V. cholerae strain N16961 containing dksA gene with its putative natural promoter cloned in pDrive; Apr KmrThis study
    pBSDA3.50.6-kb ΔdksA allele in pBluescript II KS(+); Apr KmrThis study
    pBSDA4.81.8-kb ΔdksAkan allele in pBluescript II KS(+); Apr KmrThis study
    pKDK11.9-kb ΔdksAkan allele in pKAS32; Apr KmrThis study
    pDksAVc841- bp dksA region of V. cholerae strain N16961 from pDDKW1 subcloned pBR322; AprThis study
    pDksABADV. cholerae dksA ORF cloned in pBAD24; AprThis study
    pFliABADV. cholerae fliA ORF cloned in pBAD24; AprThis study
    pRelVBADV. cholerae relV ORF cloned in pBAD24; AprThis study