Table 1

Strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)Source or reference
Strains
    E. coli JM109Cloning strain
    E. coli S17-1Conjugation strain41
    R. eutropha H16Wild-type strainDSMZ 428
    R. eutropha PHB-4PHB-negative mutant of R. eutropha H1639
    R. eutropha H16 ΔphaC1Chromosomal deletion of phaC1This study
    R. eutropha H16 ΔphaP1Chromosomal deletion of phaP135
    R. eutropha H16 ΔphaP6Chromosomal deletion of phaP6This study
    R. eutropha H16 ΔphaP7Chromosomal deletion of phaP7This study
Plasmids
    pBBR1MCS2-PphaC-eyfp-c1Universal vector for construction of fusions C terminal to eyfp under the control of the phaCAB promoter30
    pBBR1MCS2-PphaC-eyfp-n1Universal vector for construction of fusions N terminal to eyfp under the control of the phaCAB promoter31
    pBBR1MCS-2-PphaC-eyfp-phaP1C-terminal fusion of PhaP1 to eYFPThis study
    pBBR1MCS-2-PphaC-eyfp-phaP2C-terminal fusion of PhaP2 to eYFP30
    pBBR1MCS-2-PphaC-eyfp-phaP3C-terminal fusion of PhaP3 to eYFPThis study
    pBBR1MCS-2-PphaC-eyfp-phaP4C-terminal fusion of PhaP4 to eYFP30
    pBBR1MCS-2-PphaC-eyfp-phaP5C-terminal fusion of PhaP5 to eYFP30
    pBBR1MCS-2-PphaC-eyfp-phaP6C-terminal fusion of H16_B1988 to eYFPThis study
    pBBR1MCS-2-PphaC-eyfp-phaP7C-terminal fusion of H16_B2326 to eYFPThis study
    pBBR1MCS-2-PphaC-eyfp-B2296C-terminal fusion of H16_B2296 to eYFPThis study
    pBBR1MCS-2-PphaC-phaP1-eyfpN-terminal fusion of PhaP1 to eYFPThis study
    pBBR1MCS-2-PphaC-phaP2-eyfpN-terminal fusion of PhaP2 to eYFPThis study
    pBBR1MCS-2-PphaC-phaP3-eyfpN-terminal fusion of PhaP3 to eYFPThis study
    pBBR1MCS-2-PphaC-phaP4-eyfpN-terminal fusion of PhaP4 to eYFPThis study
    pBBR1MCS-2-PphaC-phaP5-eyfpN-terminal fusion of PhaP5 to eYFP31
    pBBR1MCS-2-PphaC-phaP6-eyfpN-terminal fusion of H16_B1988 to eYFPThis study
    pBBR1MCS-2-PphaC-phaP7-eyfpN-terminal fusion of H16_B2326 to eYFPThis study
    pBBR1MCS-2-PphaC-B2296-eyfpN-terminal fusion of H16_B2296 to eYFPThis study
    pBBR1MCS-2-PphaC-phaC1-eyfpN-terminal fusion of PhaC1 to eYFPThis study
    pBBR1MCS-2-PphaC-phaC1(C319A)-eyfpN-terminal fusion of PhaC1(C319A) to eYFPThis study
    pBBR1MCS-2-PphaC-phaC2-eyfpN-terminal fusion of PhaC2 to eYFPThis study
    pLO3Suicide vector; Tcr23
    pLO3 ΔphaC1Deletion vector for phaC1; fragments upstream and downstream of the gene were connected via overlap PCR and cloned between SacI and XbaI sites of pLO3This study
    pLO3 ΔphaP6Deletion vector for phaP6 (H16_B1988)This study
    pLO3 ΔphaP7Deletion vector for phaP7 (H16_B2326)This study