Table 2

Oligonucleotides used in this study

OligonucleotideSequence (5′–3′)a
C-terminal fusions to eyfp
    phaP1_C_f_XhoICCGCTCGAGGCATGATCCTCACCCCGGAACAAGTTGC
    phaP1_C_r_BamHICGGGATCCTCAGGCAGCCGTCGTCTTCTTTGC
    phaP2_C_f _XhoICCGCTCGAGGCATGACTCAGTGGACAGCAG
    phaP2_C_r_BamHICGGGATCCCTACTTTGCAGCTGCCGG
    phaP3_C_f _XhoICCGCTCGAGGCATGTCCCCTTTTATGCCCG
    phaP3_C_r_BamHICGGGATCCTTATTGCTTGGAAGCGCGG
    phaP4_C_f_XhoICCGCTCGAGGCATGACTCAGTGGTCCCCC
    phaP4_C_r_BamHICGGGATCCTTAATTTGCAGCTGCCTTTGAGG
    phaP5_C_f_XhoICCGCTCGAGGCATGGCCACGCCTCCC
    phaP5_C_r_BamHICGGGATCCCTAGCCCTTGGATTTCGGCTTG
    B1988_C_f_XhoICCGCTCGAGGCATGCAACATAAATACCCCTATGCTTGGAGG
    B1988_C_r_BamHICGGGATCCTCATGATGCACGCAGGCGCTTCG
    B2326_C_f_XhoICCGCTCGAGGCATGATGACCGCAATGCAAACCTTCTGG
    B2326_C_r_BamHICGGGATCCTCATGCCGGCCGCGGGGTTTCG
    B2296_C_f_XhoICCGCTCGAGGCATGCGTATGAAACGCCAGCCAGCATCG
    B2296_C_r_BamHICGGGATCCTCATGCGCGACGGGTCCGCTCG
N-terminal fusions to eyfp
    phaC1_N_f_NdeIGGGAATTCCATATGGCGACCGGCAAAGG
    phaC1_N_r_BamHICGGGATCCCCTGCCTTGGCTTTGACGTATCG
    phaC2_N_f_NdeIGGGAATTCCATATGAGGATCGACGACAGGACG
    phaC2_N_r_BamHICGGGATCCCCGGGTTCCAGCACGTAGGTACC
    phaP1_N_f_NdeIGGGAATTCCATATGATCCTCACCCCGGAACAAGTTGC
    phaP1_N_r_BamHICGGGATCCCCGGCAGCCGTCGTCTTCTTTGCC
    phaP2_N_f_NdeIGGGAATTCCATATGACTCAGTGGACAGCAGAGCAATGC
    phaP2_N_r_BamHICGGGATCCCCCTTTGCAGCTGCCGGAGACGC
    phaP3_N_f_XhoICCGCTCGAGCCACAGGAGATTTTGCATGTCCCCTTTTATGC
    phaP3_N_r_BamHICGGGATCCCCTTGCTTGGAAGCGCGGGGC
    phaP4_N_f_NdeIGGGAATTCCATATGACTCAGTGGTCCCCCGAGC
    phaP4_N_r_BamHICGGGATCCCCATTTGCAGCTGCCTTTGAGGCC
    phaP5_N_f_NdeIGGGAATTCCATATGGCCACGCCTCCCAATCC
    phaP5_N_r_BamHICGGGATCCCCGCCCTTGGATTTCGGCTTGAGC
    B1988_N_f_NdeIGGGAATTCCATATGCAACATAAATACCCCTATGCTTGGAGG
    B1988_N_r_BamHICGGGATCCCCTGATGCACGCAGGCGCTTCG
    B2326_N_f_NdeIGGGAATTCCATATGATGACCGCAATGCAAACCTTCTGG
    B2326_N_r_BamHICGGGATCCCCTGCCGGCCGCGGGGTTTCG
    B2296_N_f_NdeIGGGAATTCCATATGCGTATGAAACGCCAGCCAGCATCG
    B2296_N_r_BamHICGGGATCCCCTGCGCGACGGGTCCGCTCG
phaC1(C319A) eyfp fusion
    phaC1_C319A_fwdCGTGCTCGGCTTCGCCGTGGGCGGCAC
    phaC1_C319A_revGTGCCGCCCACGGCGAAGCCGAGCAC
Chromosomal deletions
    phaC1upstreamFCGATCAGAGCTCTGCGCGCGCTCGGCTTCAGCCTTGC
    phaC1upstreamRGCCGTTAATTAAGCCGGATTTGATTGTCTCTCTGCCGTCACTATTCG
    phaC1downstreamFCGGCTTAATTAACGGCCGCTTGCATGAGTGCCGGCGTGCGTCATGC
    phaC1downstreamRGCTCTAGACCCTTCCTTATTTGCGCTCGACTGCCAGCGCCACG
    phaP6upstreamFTGAAGGCGGAGCTCTCGCTCGCCATGG
    phaP6upstreamRGCCGTTAATTAAGCCGTGCACCATTTCTTTTGGTTTTCTGCGCTGG
    phaP6downstreamFCGGCTTAATTAACGGCAGGCTGCCGGGCCCGGGCGCATGC
    phaP6downstreamRGCTCTAGACCACATCGGGCCTGCGCGTGC
    phaP7upstreamFCGATCAGAGCTCACGGCGGCACCCTCTTCCTCG
    phaP7upstreamRGCCGTTAATTAAGCCGCACGGGAATCTCCGGTGGTGGGC
    phaP7downstreamFCGGCTTAATTAACGGCCCCGGCCGCCCCCGCCTTGCGC
    phaP7downstreamRGCTCTAGAGGCAGTACGCCGGGAGGCATCAGC
  • a Underlining indicates restriction sites; bold type indicates start and stop codons.