Table 1

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant phenotypeaDescription (reference)
Strains
    Escherichia coli
        BL21(DE3)CamrCells enabling high-level expression of heterologous proteins in E. coli (Stratagene)
        BacterioMatch II reporter strainKanrHost strain used for detection of protein-protein interactions in BacterioMatch II 2-hybrid system (Stratagene; catalog no. 200195)
    Pseudomonas aeruginosa PAO-1Obtained from ATCC (39)
Plasmids
    pETPA2960KanrpET-28-based plasmid used for His6-tagged PA2960 protein expression in E. coli
    pETXAC1133AmprpET-30-based plasmid used for His6-tagged XAC1133 expression in E. coli
    pET-EALPA4959KanrpET-28-based plasmid that encodes the EAL domain (aa 436–691)b of FimX (PA4959) as described previously (29)
    pET-EALXAC2398KanrpET-28-based plasmid used for His6-tagged EAL domain (aa 436–698) of XAC2398 expression in E. coli
    pET-EALXAC2398 (M487E)KanrPlasmid used for expression of M487E mutant protein
    pET-EALXAC2398 (A493E)KanrPlasmid used for expression of A493E mutant protein
    pET-EALXAC2398 (M487E L459R)KanrPlasmid used for expression of M487E L459R double mutant protein
    pET-EALXAC2398 (M487E L459R A493E)KanrPlasmid used for expression of M487E L459R A493E triple mutant protein
    pBTCmrBait plasmid used for detection of protein-protein interaction in BacterioMatch II 2-hybrid system (Agilent Technologies; catalog no. 240065)
    pBTXAC1133CmrPlasmid constructed by cloning DNA fragment encoding XAC1133 (aa 1–117) into vector pBT
    pBTPA2960CmrPlasmid constructed by cloning DNA fragment encoding PA2960 (aa 1–119) into vector pBT
    pTRGTetrrPrey plasmid used for detection of protein-protein interaction in BacterioMatch II 2-hybrid system
    pTRG-EALPA4959TetrrPlasmid constructed by cloning DNA fragment encoding EAL domain (aa 436–691) of FimX (PA4959) into vector pTRG
    pTRG-EALXAC2398TetrrPlasmid constructed by cloning DNA fragment encoding EAL domain (aa 436–698) of XAC2398 into vector pTRG
    pTRG-Gal11PTetrrPositive interaction control plasmid in BacterioMatch II 2-hybrid system, encoding a domain of mutant form of Gal11 protein
    pBT-LGF2CmrPositive interaction control plasmid in BacterioMatch II 2-hybrid system, encoding dimerization domain of Gal4 transcriptional activator protein
  • a Cmr, chloramphenicol resistance; Ampr, ampicillin resistance; Tetrr, tetracycline resistance; Kanr, kanamycin resistance.

  • b aa, amino acids.