Table 2

Thermodynamic and kinetic parameters from SPR measurements

Protein pairc-di-GMP (5 μM)KD (μM)ka (M−1 s−1)kd (s−1)
EALXAC2398-PilZXAC11330.092.6 × 1052.4 × 10−2
EALXAC2398-PilZXAC1133 +0.112.4 × 1051.4 × 10−2
EALPA4959-PilZPA2960NDaNDND
EALPA4959-PilZPA2960+NDNDND
EALXAC2398-PilZPA29600.062.4 × 1051.4 × 10−2
EALPA4959-PilZXAC1133NDNDND
EALXAC2398 (M487E)-PilZXAC11330.318.5 × 1042.6 × 10−2
EALXAC2398 (A493E)-PilZXAC1133NDNDND
EALXAC2398 (M487E L459R)-PilZXAC11330.261.2 × 1053.1 × 10−2
EALXAC2398 (M487E L459R A493E)-PilZXAC1133NDNDND
  • a ND, not determined due to weak binding and fast kinetics.