Table 1

Bacterial strains and plasmidsa

Strain or plasmidRelevant characteristicsReference or origin
Listeria monocytogenes strains
    EGDeWild type; serovar 1/2aJ. Kreft
    WSLC 1042Wild type; serovar 4bATCC 23074
    EGDeΔtagO1EGDeΔlmo0959; EGDe carrying a chromosomal deletion of lmo0959This study
    EGDeΔtagO2EGDeΔlmo2519; EGDe carrying a chromosomal deletion of lmo2519This study
    1042ΔtagO11042ΔLMO1042_0979; WSLC 1042 carrying a chromosomal deletion of LMO1042_0979This study
    1042ΔtagO21042ΔLMO1042_2492; WSLC 1042 carrying a chromosomal deletion of LMO1042_2492This study
    EGDeΔtagO1ΔtagO2::pLIV2(tagO1)EGDe with a double deletion (Δlmo0959 and Δlmo2519) and lmo0959 expressed from an IPTG-inducible promoter; Camr IPTG (+/−)bThis study
    1042ΔtagO1ΔtagO2::pLIV2(tagO1)WSLC 1042 with a double deletion (ΔLMO1042_0979 and ΔLMO1042_2492) and LMO1042_0979 expressed from an IPTG-inducible promoter; Camr IPTG (+/−)This study
Escherichia coli strains
    XL1-Blue MRF′Used for plasmid manipulations; Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)]Stratagene
    XL1-Blue MRF′ pHGFP_CBD118Used for protein expression of HGFP_CBD118; Ampr Tetr33
    XL1-Blue MRF′ pHGFP_CBD511Used for protein expression of HGFP_CBD511; Ampr Tetr43
    XL1-Blue MRF′ pHGFP_CBDP40Used for protein expression of HGFP_CBDP40; Ampr Tetr43
    XL1-Blue MRF′ pHGFP_CBDP35Used for protein expression of HGFP_CBDP35; Ampr Tetr43
Plasmids
    pKSV7Gram-negative bacterium/Gram-positive bacterium shuttle vector; thermosensitive; 6.9 kb; Camr44
    pAUL-AGram-negative bacterium/Gram-positive bacterium shuttle vector; thermosensitive; 9.2 kb; Eryr5
    pLIV2L. monocytogenes site-specific phage integration vector; IPTG-controlled expression; 6.1 kb; Camr24
    pKSV7(Δlmo0959)pKSV7 with lmo0959 flanking regions; CamrThis study
    pKSV7(Δlmo2519)pKSV7 with lmo2519 flanking regions; CamrThis study
    pKSV7(ΔLMO1042_0979)pKSV7 with LMO1042_0979 flanking regions; CamrThis study
    pKSV7(ΔLMO1042_2492)pKSV7 with LMO1042_2492 flanking regions; CamrThis study
    pAUL-A(Δlmo0959)pAUL-A with lmo0959 flanking regions; EryrThis study
    pAUL-A(ΔLMO1042_0979)pAUL-A with LMO1042_0979 flanking regions; EryrThis study
    pLIV2(Δlmo0959)pLIV2 with lmo0959 under IPTG control; CamrThis study
    pLIV2(Δlmo0979)pLIV2 with LMO1042_0979 under IPTG control; CamrThis study
  • a Genes lmo0959 and LMO1042_0979 were designated tagO1; genes lmo2519 and LMO1042_2492 were named tagO2.

  • b IPTG (+/−), IPTG was added when required to regulate the expression of tagO1 under the control of the inducible Pspac promoter.