Table 2

Binding of GFP-tagged Listeria phage endolysin CBDs to Listeria cells

L. monocytogenes strainSerovarIPTG growth conditionaPresence of WTAbBinding of CBDc
PlyP35Ply511Ply118PlyP40
EGDe1/2a+++++++++
EGDeΔtagO1+++++++++
EGDeΔtagO2+++++++++
EGDeΔtagO1ΔtagO2::pLIV2(tagO1)++++++++++
EGDeΔtagO1ΔtagO2::pLIV2(tagO1)++++++
WSLC 10424b++++
1042ΔtagO1++++
1042ΔtagO2++++
1042ΔtagO1ΔtagO2::pLIV2(tagO1)+++++
1042ΔtagO1ΔtagO2::pLIV2(tagO1)++++++
  • a When required, IPTG was added to a final concentration of 1 mM.

  • b +, normal WTA content; −, reduced or no WTA content (determined by the amount of phosphate).

  • c ++, strong binding; +, weak binding; −, no binding (estimated by fluorescence microscopy, relative to wild-type EGDe or WSLC 1042).