Table 1

Bacterial strains and plasmids

Strain or plasmidDescriptionaReference or source
Strains
    E. coli
     S17-1λpirE. coli strain for plasmid mobilization44
     DH5αE. coli strain for cloning purposes25
    C. crescentus
     NA1000Synchronizable derivative of wild-type CB1515
     MM60NA1000 strain with pRM6 translational fusion integrated in the genomeThis study
     MM61NA1000 strain with pRM7 translational fusion integrated in the genomeThis study
     MM63NA1000 strain with pRM8 translational fusion integrated in the genomeThis study
     MM62NA1000 strain with pRM9 translational fusion integrated in the genomeThis study
     MM64NA1000 strain with pRM10 translational fusion integrated in the genomeThis study
     MM8NA1000 ΔcspA31
     MM30NA1000 ΔcspBThis study
     MM26NA1000 ΔcspC4
     MM9NA1000 ΔcspD31
     MM28NA1000 ΔcspA ΔcspBThis study
Plasmids
    pGEM-T EasyCloning vector; AmprPromega
    pNPTS138Suicide vector containing oriT and sacB; KanrD. Alley
    pRKlacZ290Vector for translational fusion with lacZ gene; Tetr21
    pMR20Broad-host-range, low-copy-number vector; Tetr41
    pJBZ281pRK2-derived vector with promoterless lacZ gene; KanrM. R. K. Alley
    pEL4Fragment containing cspD promoter cloned into pRKlacZ29031
    pEL6Fragment containing cspC promoter cloned into pRKlacZ29031
    pRM1Fragment from −380 to +162 of cspA amplified using CSPA-B/CSPA-R4 transcriptionally fused to lacZ into pRKlacZ290This study
    pRM2Fragment from −196 to +162 of cspA amplified using CSPA-R5/CSPA-R4 transcriptionally fused to lacZ into pRKlacZ290This study
    pRM3Fragment from −380 to −133 upstream from cspA amplified using CSPA-B/CSPA-R8 transcriptionally fused to lacZ into pRKlacZ290This study
    pRM4Fragment from −734 to +152 of cspB amplified using CSPB-F/CSPB-R4 transcriptionally fused to lacZ into pRKlacZ290This study
    pRM5Fragment from −187 to +27 of cspB amplified using CSPB-R1/CSPB-C transcriptionally fused to lacZ into pRKlacZ290This study
    pRM6Fragment from −380 to +162 of cspA amplified using CSPA-B/CSPA-R4 translationally fused in frame to lacZ into pJBZ281This study
    pRM7Fragment from −380 to +3 of cspA amplified using CSPA-B/CSPA-R2 translationally fused in frame to lacZ into pJBZ281This study
    pRM8Fragment from −734 to +152 of cspB amplified using CSPB-F/CSPB-R4 translationally fused in frame to lacZ into pJBZ281This study
    pRM9Fragment from −734 to −93 fused to the fragment −17 to +3 of cspB amplified using CSPB-F/CSPB-R3 translationally fused in frame to lacZ into pJBZ281This study
    pRM10Fragment from −356 to +3 of cspB amplified using CSPB-A/CSPB-R2 translationally fused in frame to lacZ into pJBZ281This study
    pRM11Fragment amplified with CSPA-A/CSPA-B containing cspA gene cloned into pMR20This study
    pRM12Fragment amplified with CSPB-A/CSPB-B containing cspB gene cloned into pRM11This study
    pRM13Fragment from −380 to −90 fused to the fragment −15 to +3 of cspA amplified using CSPA-B/CSPA-R3 translationally fused in frame to lacZ into pJBZ281This study
  • a Fragment positions are relative to the ATG.