TABLE 2

E. coli strains used in this study

StrainGenotype or descriptionReference or source
MKV15bΔlpxM ΔlpxL ΔlpxP35
MLK1067 (CGSC 7701)aλ lpxM11(Ω)::Cm IN(rrnD-rrnE)1 rph-134
MLK+LpxJMLK1067 transformed with pFLAG vector carrying RT0047 (LpxJRt) or A1G_00705 (LpxJRr)This work
MLK+JH61AMLK+LpxJRt with His61 mutated to alanineThis work
MLK+JH84AMLK+LpxJRt with His84 mutated to alanineThis work
MLK+JW60AMLK+LpxJRt with Trp60 mutated to alanineThis work
MLK+JD86AMLK+LpxJRt with Asp86 mutated to alanineThis work
MLK+JD132AMLK+LpxJRt with Asp132 mutated to alanineThis work
MLK+JW172AMLK+LpxJRt with Trp172 mutated to alanineThis work
MLK+JD173AMLK+LpxJRt with Asp173 mutated to alanineThis work
  • a CGSC, Cole Genetic Stock Center, Yale University.