TABLE 1

Comparative performance of G. sulfurreducens strains lacking one cluster or containing only one clustera

Substrate% of wild type growth
ΔomcBCΔextABCDΔextEFGΔextHIJKLomcBC+extABCD+extEFG+extHIJKL+Δ5
Fe(III) citrate61.2 ± 10.5105 ± 6.662.5 ± 4.966.3 ± 2.5101.1 ± 8.499.2 ± 11.322.5 ± 2.423.8 ± 6.40.1 ± 0.6
FeIII) oxide68.9 ± 8.483.3 ± 12.187.5 ± 14.995.8 ± 24.978.8 ± 3.929.2 ± 2.660.4 ± 9.552.1 ± 3.70.1 ± 0.3
Mn(IV) oxide94.5 ± 6.495.1 ± 2.899.6 ± 3.497.9 ± 6.183.3 ± 14.126.7 ± 5.986.8 ± 6.575.6 ± 7.31.7 ± 0.9
Electrode76.5 ± 16.520.9 ± 6.0104.8 ± 2.186.3 ± 15.328.3 ± 5.2137.9 ± 9.521.2 ± 6.525.9 ± 4.221.9 ± 4.4
  • a Growth of single cytochrome conduit deletion mutants and mutants lacking all clusters except one, averaged from eight biological replicates or more and represented as percentage of wild-type growth. Averages and standard deviations are represented.