TABLE 1

Proteins with significant differences in steady-state levels between gsrN++ and wild-type strains (gsrN++/WT) measured by LC-MS/MS, with associated transcript changes

Gene nameAnnotated functionRNA-SeqaLC-MS/MSb
log2 (fold)P valueFDRlog2 (fold)P valueFDR
CCNA_02831Conserved hypothetical protein−0.57<1 × 10−170.0002−1.100.00010.0134
CCNA_03693Creatinine amidohydrolase family protein0.300.00420.0516−1.570.00430.0488
CCNA_01997Ribosome recycling factor (RRF)0.260.06870.3189−1.200.00020.0134
CCNA_03852Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide isomerase0.170.08830.3601−1.62<1 × 10−170.0093
CCNA_02388Ribose 5-phosphate isomerase−0.180.13140.4512−1.040.00020.0134
CCNA_01378Protein-l-isoaspartate O-methyltransferase0.170.14270.4707−1.090.00050.0156
CCNA_03874Carboxymethylenebutenolidase0.180.18050.5301−1.420.00080.0201
CCNA_03729Transaldolase-like protein0.180.20410.5633−1.39<1 × 10−170.0093
CCNA_01327Adenylate kinase/nucleoside-diphosphate kinase Adk0.150.22950.5976−1.930.00010.0134
CCNA_01586ABC transporter, ATP-binding protein1.150.28310.6632−1.970.00110.0232
CCNA_01624Orotate phosphoribosyltransferase0.140.31670.6997−1.210.00140.0250
CCNA_00045Inorganic pyrophosphatase0.120.35660.7311−1.270.00030.0155
CCNA_03672Superoxide dismutase−0.120.54480.8605−1.220.00020.0134
CCNA_015624-Hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxyphosphogluconate aldolase0.060.55950.8694−1.100.00030.0142
CCNA_011793′-Phosphoadenosine 5′-phosphosulfate sulfotransferase CysH−0.080.61280.8957−1.120.00280.0380
CCNA_02741Conserved hypothetical protein−0.130.68090.9288−1.45<1 × 10−170.0088
CCNA_01960Acetyl coenzyme A carboxylase biotin carboxyl carrier protein subunit−0.040.75710.9519−1.940.00040.0156
CCNA_00545Acetoacetyl coenzyme A reductase0.050.82400.9722−1.320.00040.0156
CCNA_017473-Oxoacyl-(acyl-carrier protein) reductase0.020.84550.9775−1.170.00020.0134
CCNA_01991OmpH-like outer membrane protein0.050.85800.9800−1.220.00020.0134
CCNA_03584Histidine phosphotransferase ChpT0.020.87960.9871−1.400.00030.0146
CCNA_02293Thiol:disulfide interchange protein TlpA0.020.90700.9926−1.050.00050.0156
  • a Wald's test.

  • b Student's t test.