TABLE 2

Proteins with significant differences in steady-state levels between gsrN++ and wild-type strains (gsrN++/WT) after hyperosmotic stress treatment, with associated transcript changes

Gene nameAnnotated functionRNA-SeqaLC-MS/MSb
log2 (fold)P valueFDRlog2 (fold)P valueFDR
CCNA_03839Acylamino-acid-releasing enzyme−2.46<1 × 10−17<1 × 10−17−2.030.000270.01505
CCNA_00601MoxR-like ATPase−2.34<1 × 10−17<1 × 10−17−1.280.000580.02198
CCNA_02174Multidrug resistance efflux pump−1.55<1 × 10−17<1 × 10−17−1.100.002360.03647
CCNA_02540N-Acyl-l-amino acid amidohydrolase−0.98<1 × 10−17<1 × 10−17−1.160.000570.02198
CCNA_00214TonB-dependent receptor0.746.6 × 10−78.5 × 10−6−1.740.001840.03167
CCNA_03023TonB-dependent receptor0.884.38 × 10−97.5 × 10−8−1.130.002500.03648
CCNA_03157Conserved hypothetical protein0.961.22 × 10−92.4 × 10−8−1.050.004210.04462
CCNA_03695Aldehyde dehydrogenase1.17<1 × 10−17<1 × 10−171.130.004650.04462
CCNA_00028TonB-dependent receptor1.231.8 × 10−134.9 × 10−12−1.970.000660.02198
  • a Wald's test.

  • b Student's t test. OsrP was not detected by global LC-MS/MS proteomic analysis.