TABLE 1

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsaSource or reference
Strains
    E. coli
        DH5αrecA1 endA193
        S17-1recA endA thi hsdR RP4-2 Tc::Mu::Tn7 Tpr Smr87
    Agrobacterium sp. H13-3
        P13AGROH133_08824::Tn5-110 (2589542)This study
        P24AGROH133_07337::Tn5-110 (1979068)This study
        P29AGROH133_08824::Tn5-110 (2589669)This study
        P42AGROH133_08824::Tn5-110 (2589554)This study
        P47AGROH133_07337::Tn5-110 (1979094)This study
        P113AGROH133_07337::Tn5-110 (1979094)This study
        P210AGROH133_08824::Tn5-110 (2589542)This study
        P212AGROH133_08824::Tn5-110 (2589542)This study
        P219AGROH133_07337::Tn5-110 (1979052)This study
        P222AGROH133_07337::Tn5-110 (1979193)This study
        P229AGROH133_07337::Tn5-110 (1979094)This study
        P317AGROH133_08824::Tn5-110 (2589554)This study
        P321AGROH133_08824::Tn5-110 (2589417)This study
        P326AGROH133_08824::Tn5-110 (2589542)This study
        P327AGROH133_07337::Tn5-110 (1979741)This study
        P329AGROH133_08824::Tn5-110 (2589658)This study
        P415AGROH133_07337::Tn5-110 (1979017)This study
        PR18AGROH133_13050::Tn5-110 (1669378)This study
        RU12/001Smr spontaneous streptomycin-resistant wild-type strain42
        RU12/006Smr ΔflaA ΔflaB ΔflaD42
        RU12/012Smr ΔmotA28
        RU12/015Smr ΔAGROH133_08824This study
        RU12/016Smr ΔAGROH133_07337This study
        RU12/017Smr ΔAGROH133_13050This study
Plasmids
    pBBR1MCS-3Tcr; broad-host-range vector44
    pBS429Kmr; 568-bp XbaI/SalI fragment containing an AGROH133_04228 in-frame deletion cloned into pK18mobsacBThis study
    pBS430Kmr; 568-bp XbaI/SalI fragment containing an AGROH133_07337 in-frame deletion cloned into pK18mobsacBThis study
    pBS431Tcr; 387-bp KpnI/XbaI fragment containing AGROH133_08824 cloned into pBBR1-MCS3This study
    pBS432Tcr; 813-bp KpnI/XbaI fragment containing AGROH133_07337 cloned into pBBR1-MCS3This study
    pBS461Tcr; 984-bp KpnI/XbaI fragment containing AGROH133_13050 cloned into pBBR1-MCS3This study
    pBS462Kmr; 526-bp XbaI/HindIII fragment containing an AGROH133_13050 in-frame deletion cloned into pK18mobsacBThis study
    pK18mobsacBKmr lacZ mob sacB94
    pJG110Apr Kmr Tn5-110; transposon delivery vector84
    pRK600Cmr colE1 oriV RP4tra+ RP4oriT; helper in triparental matings95
  • a Nomenclature according to Bachmann (96) and Novick et al. (97). The numbers in parentheses are the nucleotide positions of transposon insertions. Sm, streptomycin; Tc, tetracycline; Km, kanamycin; Ap, ampicillin; Cm, chloramphenicol; Tp, trimethoprim.