TABLE 1

Pilin antigenic variation frequencies of Mc8013 strains

Growth (CFU/colony)aAntigenic variation frequency (%)b of strain:
Mc8013recArecXrecQreprecJ
107c1.39NDd0.220.04NDND
108e2.740.020.540.120.010.03
  • a Strains were grown on solid medium.

  • b Antigenic variation frequency was calculated by the number of pilE variant reads divided by the total read number of each strain. All mutants were statistically different from Mc8013 parental strain (P < 0.05) using the two-tailed test of population proportion.

  • c Mc8013 and recQ strains were grown for 11 h, recX and recJ strains were grown for 13 h, and the recA and rep strains were grown for 15 h.

  • d ND, no variant reads detected.

  • e Mc8013 and rep strains were grown for 17 h, and recA, recQ, recX, and recJ strains were grown for 21 h.