TABLE 2

Strain and plasmid descriptions and sources

Strain or plasmidDescriptionReference or source
N. meningitidis strains
    8013Mc serogroup C parental strain33
    8013 recA6Contains an IPTG-inducible recA geneThis study
    8013 recQ::erm recA6The recQ gene is replaced by the erm antibiotic resistant cassetteThis study
    8013 recJ::kan recA6The recJ gene is replaced by the kan antibiotic resistant cassetteThis study
    8013 recX::erm recA6The recX gene is replaced by the erm antibiotic resistant cassetteThis study
    8013 rep::kan recA6The rep gene is replaced by the kan antibiotic resistant cassetteThis study
    8013 Spr recA6Spontaneous Spc-resistant mutant, used for DNA transformation assayThis study
    Gc FA1090 recQ::ermThe Gc recQ gene is insertionally disrupted by Erm14
Plasmids
    precXKOermPlasmid to knock out recX gene, Ermr31
    precJ::KanPlasmid to knock out recJ gene, KanrThis study
    prep::KanPlasmid to knock out rep gene, KanrThis study
    precQ::ermPlasmid to knock out recQ gene, ErmrThis study