Table 2

Summary of phenotypes associated with M. tuberculosis espA::Tn strains expressing EspD variants

PlasmidEsxA secretionRelative EspA level (%)aRelative EspD level (%)bEspD secretion
pMD31No00No
pMDespACDYes100100Yes
pMDespACDstopNo330No
pMDespACDW19RYes9859No
pMDespACDW27RYes10046No
pMDespACDP31AYes6566Yesd
pMDespACDR138ANo725UNc
pMDespACDW150RYes4464No
pMDespACDΔ8CTYes9284Yes
pMDespACDΔ16CTYes8017UN
pMDespACDΔ30CTNo4119UN
  • a Indicated as a percentage of espA::Tn plus pMDespACD expressing wild-type EspA.

  • b Indicated as a percentage of espA::Tn plus pMDespACD expressing wild-type EspD.

  • c UN, unknown, either below detection limits or failure to secrete (see the text).

  • d Secreted but at a lower level compared to espA::Tn plus pMDespACD expressing wild-type EspD. Data are representative of results from three independent experiments.