TABLE 1

Intracellular concentration of pGpG following complementation of PA14 Δorn strains

StrainConcn (mean ± SD) (μM)a
pGpGc-di-GMP
Wild-type PA14/pMMB2.2 ± 0.40.016 ± 0.008
Δorn mutant/pMMB17.4 ± 3.70.58 ± 0.10
Δorn mutant/pMMB-ornPa5.2 ± 1.40.028 ± 0.013
Δorn mutant/pMMB-ornVc6.5 ± 1.80.017 ± 0.011
Δorn mutant/pMMB-nrnABs3.6 ± 1.40.022 ± 0.016
Δorn mutant/pMMB-nrnABs3.3 ± 1.20.024 ± 0.016
Δorn mutant/pMMB-nrnBBs2.5 ± 1.10.023 ± 0.011
Δorn mutant/pMMB-nrnCCc2.4 ± 1.10.020 ± 0.001
  • a From 3 experiments, calculated assuming the volume of a single bacterium equals 4.3 × 10−1 fl (9).